HIV-1 Tat Specific Factor 1 (HTATSF1) (AA 322-437) antibody

Details for Product No. ABIN968708
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Antigen
Synonyms TAT-SF1, TATSF1, dJ196E23.2, RGD1563377, zgc:66252, MGC98898, HTATSF1, DKFZp468B086
Epitope
AA 322-437
(11), (10), (9), (4), (3), (2), (1)
Reactivity
Human
(34), (6), (6), (3)
Host
Mouse
(29), (5), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunofluorescence (IF)
(32), (19), (5), (4), (3), (1), (1)
Pubmed 3 references available
Catalog no. ABIN968708
Quantity 50 µg
Price
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Immunogen Human Tat-SF1
Clone Ghr2
Isotype IgG2a
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name Tat-SF1
Background Tat protein is expressed early in the viral life cycle of the human immunodeficiency virus (HIV), and is involved in viral replication and progression to disease. Tat binds to RNA in the transactivation response element located at the 5’ end of the nascent viral transcript. Unlike DNA transcription activators, Tat promotes transcription by binding RNA and increasing the process of elongation by RNA polymerase II. Several proteins that facilitate Tat activation include Tat specific factor (Tat-SF1), elongation factor (P-TEFb), and TFIIH. Tat-SF1 is a phosphoprotein that binds the active form of Tat. Tat-SF1 contains two RNA recognition motifs (RRN) in the N-terminal regon, and a highly acidic carboxyl terminal motif. In nuclear extracts from HeLa cells, Tat-SF1 is associated with hSPT5, RAP30, and Pol II. Immunodepletion of Tat-SF1 and hSPT5 from nuclear extracts demonstrates that both are required for Tat activation, while overexpression of Tat-SF1 and hSPT5 stimulates transcriptional activity of Tat, in vivo. Thus, Tat-SF1 may associate with several other elongation and transcription factors to regulate Tat-specific transcription activity. This antibody is routinely tested by western blot analysis.
Synonyms: Tat Specific Factor-1
Molecular Weight 140 kDa
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-HIV-1 Tat Specific Factor 1 (HTATSF1) (AA 322-437) antibody Western blot analysis of Tat-SF1 on a Jurkat cell lysate (Human T-cell leukemia, ATCC TIB-152). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- Tat-SF1 antibody.
anti-HIV-1 Tat Specific Factor 1 (HTATSF1) (AA 322-437) antibody (2) Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma, ATCC CCL-2.2).
Product cited in: Zhou, Sharp: "Tat-SF1: cofactor for stimulation of transcriptional elongation by HIV-1 Tat." in: Science (New York, N.Y.), Vol. 274, Issue 5287, pp. 605-10, 1996 (PubMed).

Parada, Roeder: "A novel RNA polymerase II-containing complex potentiates Tat-enhanced HIV-1 transcription." in: The EMBO journal, Vol. 18, Issue 13, pp. 3688-701, 1999 (PubMed).

Kim, Yamaguchi, Wada et al.: "Tat-SF1 protein associates with RAP30 and human SPT5 proteins." in: Molecular and cellular biology, Vol. 19, Issue 9, pp. 5960-8, 1999 (PubMed).

Hosts (29), (5), (1)
Reactivities (34), (6), (6), (3)
Applications (32), (19), (5), (4), (3), (1), (1)
Conjugates (2), (2), (2), (2), (2), (2)
Epitopes (11), (10), (9), (4), (3), (2), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

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Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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