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Adaptor-Related Protein Complex 4, epsilon 1 Subunit (AP4E1) (AA 685-793) antibody

Details for Product No. ABIN968713, Supplier: Login to see
Antigen
  • CPSQ4
  • SPG51
  • RGD1562199
  • 2310033A20Rik
  • 9930028M04Rik
  • AV087807
Epitope
AA 685-793
2
1
1
1
Reactivity
Human
5
Host
Mouse
3
2
Clonality (Clone)
Monoclonal ()
Application
Immunofluorescence (IF), Western Blotting (WB)
5
4
3
1
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Immunogen Human Adaptin epsilon aa. 685-793
Clone 32-Adaptin epsilon
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Adaptin epsilon (AP4E1 Antibody Abstract)
Background Sorting of integral membrane proteins is mediated by vesicular trafficking between a variety of organelles. Two sorting signals are tyrosine-based and dileucine-based signals that interact with heterotetrameric adaptor protein complexes (AP-1, AP-2, AP-3, and AP-4), which are associated with the vesicle coats. These coatomers contain two large adaptin proteins (gamma, alpha, sigma, epsilon and beta1, beta2, beta3, beta4 respectively) that are noncovalently linked to one medium chain (µ1, µ2, µ3, µ4 respectively) and one small chain (sigma1, sigma2, sigma3, sigma4 respectively). The AP-1 and AP-3 complexes are involved in protein sorting from the TGN and endosomes, while AP-2 adaptor complexes are involved in clathrin-mediated endocytosis. AP-4 is associated with non-clathrin coated vesicles in the region of the TGN. This localization is disrupted by brefeldin A, indicating that AP-4 membrane attachment is regulated by small GTPases. The µ4 subunit of the AP-4 complex interacts with tyrosine-based signals on LAMP-2 during targeting to the endosomal-lysosomal system. Thus, AP-4 is a less abundant AP complex that may be important for vesicle trafficking from the Golgi to the endosomal-lysosomal system.
Molecular Weight 127 kDa
Pathways
Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Adaptor-Related Protein Complex 4, epsilon 1 Subunit (AP4E1) (AA 685-793) antibody (ABIN968713) Western blot analysis of Adaptin epsilon on SK-MEL5 lysate. Lane 1: 1:250, lane 2: 1:...
Product cited in: Aguilar, Boehm, Gorshkova et al.: "Signal-binding specificity of the mu4 subunit of the adaptor protein complex AP-4." in: The Journal of biological chemistry, Vol. 276, Issue 16, pp. 13145-52, 2001 (PubMed).

Hirst, Bright, Rous et al.: "Characterization of a fourth adaptor-related protein complex." in: Molecular biology of the cell, Vol. 10, Issue 8, pp. 2787-802, 1999 (PubMed).

DellAngelica, Mullins, Bonifacino: "AP-4, a novel protein complex related to clathrin adaptors." in: The Journal of biological chemistry, Vol. 274, Issue 11, pp. 7278-85, 1999 (PubMed).