serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1 (SERPINE1) (AA 207-329) antibody
|Synonyms||PAI, PAI1, PAI-1, PLANH1, SERPINE1, MGC154415, si:ch211-138a11.1, Planh1, Pai1, PAI1A, Planh, Pai1aa, RATPAI1A|
Alternatives Western Blotting (WB)
|3 references available|
|Price||Product not available in this region.|
|Cross-Reactivity||Dog (Canine), Rat (Rattus), Mouse (Murine), Chicken|
|Description||Fibrinolysis is regulated by the plasminogen activators, tissue plasminogen activator (tPA) and urokinase PA (uPA), and by the plasminogen activator-inhibitors (PAIs). Two PAIs include the serpin family members, PAI-1 and PAI-2. PAI-1 is a glycoprotein found in plasma, platelets, endothelial cells, hepatoma cells, and fibrosarcoma cells. Thrombin, endotoxin, and IL-1 induce PAI-1 synthesis in endothelial cells, which is where the major portion of plasma PAI-1 is produced. PAI-2 is a glycoprotein expressed in placenta and monocyte macrophages. The uPA/plasmin system may play a key role in cancer progression through degradation of the extracellular matrix during tumor cell migration. Paradoxically, high levels of PAI-1 are also predictive of poor prognosis of cancer patients. This finding may be a result of the role of plasmin proteolysis in the prevention of tumor vessel assembly. PAI-1 can promote tumor angiogenesis, and the mechanism may involve PAI-induced regulation of plasmin proteolysis during tumor angiogenesis. Thus, PAI-1 is a serpin protease inhibitor that is important for the regulation of plasmin proteolysis during fibrinolysis and extracellular matrix degradation.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||47 kDa|
Related Products: ABIN968536, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20° C.|
|Restrictions||For Research Use only|
|Western blot analysis of PAI-1 on human endothelial cell lysate. Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10000 of anti-PAI-1 antibody.|
Ginsburg, Zeheb, Yang et al.: "cDNA cloning of human plasminogen activator-inhibitor from endothelial cells." in: The Journal of clinical investigation, Vol. 78, Issue 6, pp. 1673-80, 1986 (PubMed).
Bajou, Masson, Gerard et al.: "The plasminogen activator inhibitor PAI-1 controls in vivo tumor vascularization by interaction with proteases, not vitronectin. Implications for antiangiogenic strategies." in: The Journal of cell biology, Vol. 152, Issue 4, pp. 777-84, 2001 (PubMed).
Cai, Li, Goette et al.: "Downregulation of endocardial nitric oxide synthase expression and nitric oxide production in atrial fibrillation: potential mechanisms for atrial thrombosis and stroke." in: Circulation, Vol. 106, Issue 22, pp. 2854-8, 2002 (PubMed).