Phosphatidylinositol Transfer Protein, Membrane-Associated 1 (PITPNM1) antibody

Antigen: Phosphatidylinositol Transfer Protein, Membrane-Associated 1 (PITPNM1)

Synonyms: Rd9, NIR2, RDGB, DRES9, RDGB1, RDGBA, PITPNM, RDGBA1, FLJ44997, RdgB, mpt-1, Pitpnm, R75447, MGC95131

Clonality: Monoclonal (8)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN968720

Additional Information

Alternative name: PITPNM

Immunogen: Mouse PITPnm

Cross-Reactivity: Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 8

Description: PITPs (phosphatidylinositol transfer protein) are abundant cytosolic or transmembrane proteins that are required for phospholipase C (PLC) signaling and vesicular trafficking. All of the mammalian PITPs are homologous to Drosophila retinal degeneration B (rdgB) proteins, which are PITPs that are involved in retinal and olfactory neurosensory signaling, and mutations in these proteins lead to retinal degeneration. There are at least 5 mammalian PITPs, three cytosoloic PITPs, PITPalpha, PITPbeta, and MrdgBbeta, and two transmembrane PITPs: PITPnm (Nir2/MrdgBalpha) and Nir3. PITPalpha and beta participate in PLC signaling, mediated by the ability of PITP to deliver PI to the signaling complex containing PI4-Kinase, PIP-5-Kinase, and the activated receptor. PITPnm contains an N-terminal PITP domain adjacent to a Ca2+-binding domain (CBD), and a C-terminal PYK2 binding domain. PITPnm mRNA is widely expressed with the highest expression in brain, and PITPnm protein is found in the ER and Golgi. Thus, PITPnm may be important for phosphoinositide synthesis in the Golgi, and may interact with PYK2 during activation of PI-4 signaling pathways.
Synonyms: Phosphatidylinositol transfer protein, Nir2, MrdgBalpha

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 170 kDa

Comments:

Related Products: ABIN968545, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of PITPnm on a rat cerebrum lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-PITPnm antibody.

Publications

Product: Aikawa, Hara, Watanabe: "Molecular cloning and characterization of mammalian homologues of the Drosophila retinal degeneration B gene." in: Biochemical and biophysical research communications, Vol. 236, Issue 3, pp. 559-64, 1997 (PubMed).

Lev, Hernandez, Martinez et al.: "Identification of a novel family of targets of PYK2 related to Drosophila retinal degeneration B (rdgB) protein." in: Molecular and cellular biology, Vol. 19, Issue 3, pp. 2278-88, 1999 (PubMed).

Aikawa, Kuraoka, Kondo et al.: "Involvement of PITPnm, a mammalian homologue of Drosophila rdgB, in phosphoinositide synthesis on Golgi membranes." in: The Journal of biological chemistry, Vol. 274, Issue 29, pp. 20569-77, 1999 (PubMed).

Fullwood, dos Santos, Hsuan: "Cloning and characterization of a novel human phosphatidylinositol transfer protein, rdgBbeta." in: The Journal of biological chemistry, Vol. 274, Issue 44, pp. 31553-8, 1999 (PubMed).