Cullin 1 (CUL1) (AA 56-246) antibody

Antigen: Cullin 1 (CUL1)

Synonyms: LOC100282292, CUL1, Cullin-1, DKFZp459I053, MGC149834, MGC149835, Cul1, zgc:55747, zgc:77500, wu:fc13a10

Binding Site: AA 56-246

Clonality: Monoclonal (19)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN968721

Additional Information

Alternative name: CUL-1

Immunogen: Mouse CUL-1

Cross-Reactivity: Human, Rat (Rattus), Dog (Canine), Chicken

Format: Liquid

Isotype: IgG1

Clone: 19

Description: The cullins are a family of proteins that are integral to cell cycle regulation. Members of this family include human Cul proteins, C.elegans Ce-Cul-1, and S. cerevisiae Cdc53. Proper control of cell cycle progression is essential for the prevention of tumorigenesis. Mutant forms of both Ce-Cul-1 and Cdc53 have been implicated in the oncogenic process. The human Cul family of proteins, Cul-1, -2, -3, -4A, -4B, and -5, have also been implicated in oncogenic processes. Abnormal nuclear localization of Cul-2 is seen in the VHL (von Hippel-Lindau) syndrome, which includes development of a variety of cancers. Cul-3 is widely expressed in normal cells, and has increased expression in colon cancer cells. Cul-3 can associate with cyclin E in mammalian cells, and overexpression of Cul-3 targets cyclin E for ubiquination. Cul-1 associates with the ubiquination-conjugating complex, cyclin A/CDC34/p45[SKP2]/p19[SKP1]. In addition, Cul-1 interacts with p19[SKP1], E2 ubiquination-conjugating enzyme, and FWD1 to facilitate ubiquitin degradation of IkappaBalpha, beta.catenin, and Vpu proteins. Thus, Cul-1, as well as other cullin family members, may have important roles in cycle-dependent ubiquitin degradation.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 90 kDa

Comments:

Related Products: ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Restrictions: For Research Use only

Images

Western blot analysis of CUL-1 on a P19 cell lysate (Mouse embryonal carcinoma, ATCC CRL-1825) . Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- CUL-1 antibody.

Publications

Product: Kipreos, Lander, Wing et al.: "cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family." in: Cell, Vol. 85, Issue 6, pp. 829-39, 1996 (PubMed).

Lisztwan, Marti, Sutterlüty et al.: "Association of human CUL-1 and ubiquitin-conjugating enzyme CDC34 with the F-box protein p45(SKP2): evidence for evolutionary conservation in the subunit composition of the CDC34-SCF pathway." in: The EMBO journal, Vol. 17, Issue 2, pp. 368-83, 1998 (PubMed).

Hatakeyama, Kitagawa, Nakayama et al.: "Ubiquitin-dependent degradation of IkappaBalpha is mediated by a ubiquitin ligase Skp1/Cul 1/F-box protein FWD1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 7, pp. 3859-63, 1999 (PubMed).