CTBP1 antibody (AA 345-441)

Catalog No. ABIN968722

Product Details anti-CTBP1 Antibody

Target: CTBP1 (C-terminal Binding Protein 1 (CTBP1))

Binding Specificity: AA 345-441

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Human, Rat (Rattus), Chicken, Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse CtBP1 aa. 345-441

Clone: 3-CtBP1

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-CTBP1 antibody (ABIN968722)

Hildebrand, Soriano: "Overlapping and unique roles for C-terminal binding protein 1 (CtBP1) and CtBP2 during mouse development." in: Molecular and cellular biology, Vol. 22, Issue 15, pp. 5296-307, (2002) (PubMed).

Muraoka, Ichikawa, Shi, Okumura, Taira, Higuchi, Hirano, Hibi, Miki: "Kheper, a novel ZFH/deltaEF1 family member, regulates the development of the neuroectoderm of zebrafish (Danio rerio)." in: Developmental biology, Vol. 228, Issue 1, pp. 29-40, (2001) (PubMed).

Furusawa, Moribe, Kondoh, Higashi: "Identification of CtBP1 and CtBP2 as corepressors of zinc finger-homeodomain factor deltaEF1." in: Molecular and cellular biology, Vol. 19, Issue 12, pp. 8581-90, (2000) (PubMed).

Schaeper, Boyd, Verma, Uhlmann, Subramanian, Chinnadurai: "Molecular cloning and characterization of a cellular phosphoprotein that interacts with a conserved C-terminal domain of adenovirus E1A involved in negative modulation of oncogenic transformation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 23, pp. 10467-71, (1995) (PubMed).

Target Details for CTBP1

Target: CTBP1 (C-terminal Binding Protein 1 (CTBP1))

Alternative Name: CtBP1 (CTBP1 Products)

Synonyms: BARS antibody, CtBP1-L antibody, CtBP1-S antibody, CtBP3/BARS antibody, D4S115h antibody, D5H4S115 antibody, D5H4S115E antibody, 50-kDaBFA-inducedADP-ribosylatedsubstrate antibody, BARS-50 antibody, Bars antibody, wu:fi43a11 antibody, CtBP antibody, ctbp1-A antibody, xctbp1 antibody, bars antibody, CTBP1 antibody, C-terminal binding protein 1 antibody, C-terminal binding protein 1 L homeolog antibody, CTBP1 antibody, Ctbp1 antibody, ctbp1 antibody, ctbp1.L antibody

Background: Transcriptional repression is critical for genetic regulation of many cellular proteins, and involves either a transcription regulator that has an intrinsic repressor domain, or a transcription regulator that contains a binding site in the regulatory domain where a corepressor can bind. C-terminal binding proteins (CtBP1 and CtBP2) are a class of corepressors that interact with a subset of transcription factors through a PLDLSL sequence motif. CtBP1 and CtBP2 are 80% homologous, and both are expressed at high levels in embryonic tissues. However, CtBP1 is expressed higher in adult tissues, and is more widely expressed in embryonic and adult tissues. Both CtBP1 and CtBP2 bind the deltaEF1 zinc finger-homeodomain transcription factor, and enhance transcriptional repression via interaction with the PLDLSL-sequence in deltaEF1. CtBP1 and CtBP2 bind other zinc finger transcription factors, including Kheper and BKLF. In addition, CtBP2 interacts with other transcription factors, such as hFOG-2, Evi-1, AREB6, and ZEB. Thus, CtBP1 and CtBP2 may be important corepressors for a variety of transcriptional factors. This antibody is routinely tested by western blot analysis.
Synonyms: C-terminal Binding Protein-1

Molecular Weight: 48 kDa

Pathways: Retinoic Acid Receptor Signaling Pathway