TAF4 antibody (AA 454-565)

Catalog No. ABIN968728

Product Details anti-TAF4 Antibody

Target: TAF4 (TAF4 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 135kDa (TAF4))

Binding Specificity: AA 454-565

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This TAF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human TAF[II]135 aa. 454-565

Clone: 22-TAF[II]135

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-TAF4 antibody (ABIN968728)

Freiman, Albright, Chu, Zheng, Liang, Sha, Tjian: "Redundant role of tissue-selective TAF(II)105 in B lymphocytes." in: Molecular and cellular biology, Vol. 22, Issue 18, pp. 6564-72, (2002) (PubMed).

Shimohata, Nakajima, Yamada, Uchida, Onodera, Naruse, Kimura, Koide, Nozaki, Sano, Ishiguro, Sakoe, Ooshima, Sato, Ikeuchi, Oyake, Sato, Aoyagi, Hozumi, Nagatsu, Takiyama, Nishizawa, Goto, Kanazawa et al.: "Expanded polyglutamine stretches interact with TAFII130, interfering with CREB-dependent transcription. ..." in: Nature genetics, Vol. 26, Issue 1, pp. 29-36, (2000) (PubMed).

Saluja, Vassallo, Tanese: "Distinct subdomains of human TAFII130 are required for interactions with glutamine-rich transcriptional activators." in: Molecular and cellular biology, Vol. 18, Issue 10, pp. 5734-43, (1998) (PubMed).

Mengus, May, Carré, Chambon, Davidson: "Human TAF(II)135 potentiates transcriptional activation by the AF-2s of the retinoic acid, vitamin D3, and thyroid hormone receptors in mammalian cells." in: Genes & development, Vol. 11, Issue 11, pp. 1381-95, (1997) (PubMed).

Target Details for TAF4

Target: TAF4 (TAF4 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 135kDa (TAF4))

Alternative Name: TAF II135 (TAF4 Products)

Synonyms: CG5444 antibody, Dmel\\CG5444 antibody, SR3-3 antibody, SY3-2 TAF110 antibody, TAF antibody, TAF110 antibody, TAF4 antibody, TAFII110 antibody, TAF[II]110 antibody, TAF[[II]] antibody, TAF[[II]]110 antibody, TFIID antibody, TFIID 110 antibody, Taf110 antibody, Taf[[II]]110 antibody, d110 antibody, dTAF4 antibody, dTAFII110 antibody, dTAF[[II]]110 antibody, dmTAF4 antibody, l(3)72Dj antibody, p110 antibody, taf110 antibody, TAF2C antibody, TAF2C1 antibody, TAF4A antibody, TAFII130 antibody, TAFII135 antibody, TBP-associated factor 4 antibody, Taf4p antibody, TATA-box binding protein associated factor 4 antibody, TATA-box binding protein associated factor 4 L homeolog antibody, Taf4 antibody, TAF4 antibody, taf4.L antibody

Background: Tightly associated factors (TAFs) play an essential role in transcriptional activation through their interaction with a variety of activators. TAF[II]135 (a.k.a. TAF[II]130) is a human homologue of Drosophila TAF[II]110, the first TAF shown to have coactivator activity. TAF[II]135 contains multiple glutamine-rich regions, as well as a coactivator domain (CAD). The glutamine-rich regions of TAF[II]135 facilitate interaction with Sp1 and CREB, which leads to enhancement of both Sp1- and CREB-mediated transcription. In addition, TAF[II]135 can potentiate transcriptional stimulation by AF-2 of the retinoic acid, thyroid hormone, and vitamine D3 receptors. However, TAF[II]135 does not interact with the AF-2s of the estrogen and retinoid X receptors. Interestingly, TAF[II]135 enhancement of CREB transcriptional activity may be disrupted by expanded polyglutamine (CAG) repeats found in at least eight different neurodegenerative disorders. Thus, TAF[II]135 may have important coactivator activities in many different transcription-regulating cell signaling pathways, and interference of TAF[II]135 activity by CAG repeats may be a common mechanism of specific types of neuropathologies.

Molecular Weight: 135 kDa