Dystroglycan 1 (DAG1) (AA 655-767) antibody

Antigen: Dystroglycan 1 (DAG1)

Binding Site: AA 655-767

Clonality: Monoclonal (56)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN968734

Additional Information

Alternative name: beta-Dystroglycan

Immunogen: Human beta-Dystroglycan

Format: Liquid

Isotype: IgG2a

Clone: 56

Description: Formation of the neuromuscular junction (NMJ) requires interaction between specific extracellular matrix proteins, intracellular cytoskeletal elements, and clustering of specific neurotransmitter receptors. Dystroglycans, alpha-dystroglycan and beta-dystroglycan, are two members of the dystrophin-associated complex, an essential element of NMJs. These proteins are encoded by the same gene, but posttranslationally cleaved to produce a 156 kDa extracellular peripheral membrane protein, alpha-dystroglycan, and a 43 kDa transmembrane protein, beta-dystroglycan. Dystroglycans are expressed highest in heart and muscle, but are also found in non-muscle tissues. alpha-dystroglycan may interact with agrin to facilitate AChR clustering at NMJs, and has been implicated as a laminin receptor. beta-dystroglycan recruits dystrophin to the sarcolemma, and interactions between beta-dystroglycan and caveolin-3 may regulate this recruitment. Mice deficient in dystroglycans have severly disorganized NMJs, and have reductions in the concentration of laminin, perlecan, and AChE at the synaptic basement membrane of NMJs. Thus, dystroglycans may have important extracellular and intracellular roles during NMJ assembly.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 43-50 kDa

Comments:

Related Products: ABIN968587, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Restrictions: For Research Use only

Images

Western blot analysis of beta-Dystroglycan on a HepG2 cell lysate (Human hepatocellular carcinoma, ATCC HB-8065). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-human beta-Dystroglycan antibody.

Publications

Product: Ibraghimov-Beskrovnaya, Milatovich, Ozcelik et al.: "Human dystroglycan: skeletal muscle cDNA, genomic structure, origin of tissue specific isoforms and chromosomal localization." in: Human molecular genetics, Vol. 2, Issue 10, pp. 1651-7, 1994 (PubMed).

Sotgia, Lee, Das et al.: "Caveolin-3 directly interacts with the C-terminal tail of beta -dystroglycan. Identification of a central WW-like domain within caveolin family members." in: The Journal of biological chemistry, Vol. 275, Issue 48, pp. 38048-58, 2000 (PubMed).

Jacobson, Côté, Rossi et al.: "The dystroglycan complex is necessary for stabilization of acetylcholine receptor clusters at neuromuscular junctions and formation of the synaptic basement membrane." in: The Journal of cell biology, Vol. 152, Issue 3, pp. 435-50, 2001 (PubMed).