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Dystroglycan antibody (Dystroglycan 1) (AA 655-767)

Details for Product anti-DAG1 Antibody No. ABIN968734, Supplier: Login to see
Antigen
  • D9Wsu13e
  • DG
  • Dp427
  • Dp71
  • 156DAG
  • A3a
  • AGRNR
  • DAG
  • MDDGC7
  • MDDGC9
  • RAB7
Epitope
AA 655-767
22
15
13
8
8
4
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
90
41
37
6
4
4
1
1
1
1
1
1
1
1
Host
Mouse
61
16
14
3
Clonality (Clone)
Monoclonal ()
Conjugate
This Dystroglycan antibody is un-conjugated
5
5
5
3
3
3
2
2
2
2
2
2
2
2
2
Application
Immunofluorescence (IF), Western Blotting (WB)
60
42
25
22
15
3
3
3
2
1
1
1
Supplier
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Immunogen Human beta-Dystroglycan aa. 655-767
Clone 56-beta
Isotype IgG2a
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name beta-Dystroglycan (DAG1 Antibody Abstract)
Background Formation of the neuromuscular junction (NMJ) requires interaction between specific extracellular matrix proteins, intracellular cytoskeletal elements, and clustering of specific neurotransmitter receptors. Dystroglycans, alpha-dystroglycan and beta-dystroglycan, are two members of the dystrophin-associated complex, an essential element of NMJs. These proteins are encoded by the same gene, but posttranslationally cleaved to produce a 156 kDa extracellular peripheral membrane protein, alpha-dystroglycan, and a 43 kDa transmembrane protein, beta-dystroglycan. Dystroglycans are expressed highest in heart and muscle, but are also found in non-muscle tissues. alpha-dystroglycan may interact with agrin to facilitate AChR clustering at NMJs, and has been implicated as a laminin receptor. beta-dystroglycan recruits dystrophin to the sarcolemma, and interactions between beta-dystroglycan and caveolin-3 may regulate this recruitment. Mice deficient in dystroglycans have severly disorganized NMJs, and have reductions in the concentration of laminin, perlecan, and AChE at the synaptic basement membrane of NMJs. Thus, dystroglycans may have important extracellular and intracellular roles during NMJ assembly.
Molecular Weight 43-50 kDa
Pathways
Comment

Related Products: ABIN968587, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Dystroglycan antibody (Dystroglycan 1) (AA 655-767) (ABIN968734) Western blot analysis of beta-Dystroglycan on a HepG2 cell lysate (Human hepatocellul...
Product cited in: Jacobson, Côté, Rossi et al.: "The dystroglycan complex is necessary for stabilization of acetylcholine receptor clusters at neuromuscular junctions and formation of the synaptic basement membrane." in: The Journal of cell biology, Vol. 152, Issue 3, pp. 435-50, 2001 (PubMed).

Sotgia, Lee, Das et al.: "Caveolin-3 directly interacts with the C-terminal tail of beta -dystroglycan. Identification of a central WW-like domain within caveolin family members." in: The Journal of biological chemistry, Vol. 275, Issue 48, pp. 38048-58, 2000 (PubMed).

Ibraghimov-Beskrovnaya, Milatovich, Ozcelik et al.: "Human dystroglycan: skeletal muscle cDNA, genomic structure, origin of tissue specific isoforms and chromosomal localization." in: Human molecular genetics, Vol. 2, Issue 10, pp. 1651-7, 1994 (PubMed).