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SMARCB1 antibody (AA 257-359)

SMARCB1 Reactivity: Human, Mouse, Rat WB, IF Host: Mouse Monoclonal 25-BAF47 unconjugated
Catalog No. ABIN968741
  • Target See all SMARCB1 Antibodies
    SMARCB1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily B, Member 1 (SMARCB1))
    Binding Specificity
    • 16
    • 11
    • 8
    • 6
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 257-359
    Reactivity
    • 90
    • 57
    • 26
    • 7
    • 7
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    Human, Mouse, Rat
    Host
    • 80
    • 10
    Mouse
    Clonality
    • 72
    • 18
    Monoclonal
    Conjugate
    • 47
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This SMARCB1 antibody is un-conjugated
    Application
    • 52
    • 34
    • 15
    • 15
    • 13
    • 13
    • 9
    • 8
    • 7
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF)
    Cross-Reactivity
    Rat (Rattus), Human
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Mouse BAF47 aa. 257-359
    Clone
    25-BAF47
    Isotype
    IgG2a
    Top Product
    Discover our top product SMARCB1 Primary Antibody
  • Comment

    Related Products: ABIN968540, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20° C.
  • Guidi, Sands, Zambrowicz, Turner, Demers, Webster, Smith, Imbalzano, Jones: "Disruption of Ini1 leads to peri-implantation lethality and tumorigenesis in mice." in: Molecular and cellular biology, Vol. 21, Issue 10, pp. 3598-603, (2001) (PubMed).

    Bruder, Dumanski, Kedra: "The mouse ortholog of the human SMARCB1 gene encodes two splice forms." in: Biochemical and biophysical research communications, Vol. 257, Issue 3, pp. 886-90, (1999) (PubMed).

    Kalpana, Marmon, Wang, Crabtree, Goff: "Binding and stimulation of HIV-1 integrase by a human homolog of yeast transcription factor SNF5." in: Science (New York, N.Y.), Vol. 266, Issue 5193, pp. 2002-6, (1995) (PubMed).

  • Target
    SMARCB1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily B, Member 1 (SMARCB1))
    Alternative Name
    BAF47 (SMARCB1 Products)
    Synonyms
    SMARCB1 antibody, BAF47 antibody, INI1 antibody, MRD15 antibody, RDT antibody, RTPS1 antibody, SNF5 antibody, SNF5L1 antibody, Sfh1p antibody, Snr1 antibody, hSNFS antibody, AU020204 antibody, Baf47 antibody, Ini1 antibody, SNF5/INI1 antibody, Snf5 antibody, fc51b01 antibody, smarcb1 antibody, wu:fc51b01 antibody, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 antibody, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 S homeolog antibody, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1b antibody, SMARCB1 antibody, Smarcb1 antibody, smarcb1.S antibody, smarcb1b antibody
    Background
    SWI/SNF complexes facilitate gene activation and transcription factor binding by altering repressive chromatin structures in an ATP-dependent manner. In mammals, SWI/SNF complexes are present in mutliple forms that include 9-12 BRG1-associated factors (BAFs) per complex. These BAF proteins range in molecular weight from 47 to 250 kDa. BAF47/SNF5 (SMARCB1/Ini1) complexes with BRG1- and BRM-containing SWI/SNF complexes. BAF47/SNF5 mRNA is widely expressed in cell lines and tissues, and BAF47/SNF5 gene is mutated in many human tumors. The latter is indicative of a tumor suppressor role for BAF47/SNF5. In addition, mice deficient for BAF47 die early in embryogenesis, while BAF47/SNF5 heterozygous mice display a variety of tumors in the soft tissues of the head and neck. BAF47/SNF5 also binds the HIV-1 integrase and stimulates integrase-mediated DNA joining activity. Thus, BAF47/SNF5 is a component of SWI/SNF complexes that may be critical for normal development and tumor suppression, but may also be a protein utilized for viral DNA integration into host DNA. This antibody is routinely tested by western blot analysis.
    Synonyms: SNF5
    Molecular Weight
    47 kDa
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