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HIP1R antibody (AA 560-772)

HIP1R Reactivity: Human, Rat, Mouse, Dog WB, IF Host: Mouse Monoclonal 44-Hip1R unconjugated
Catalog No. ABIN968746
  • Target See all HIP1R Antibodies
    HIP1R (Huntingtin Interacting Protein 1 Related (HIP1R))
    Binding Specificity
    • 9
    • 8
    • 7
    • 6
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 560-772
    Reactivity
    Human, Rat, Mouse, Dog
    Host
    • 48
    • 5
    • 1
    Mouse
    Clonality
    • 51
    • 3
    Monoclonal
    Conjugate
    • 26
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HIP1R antibody is un-conjugated
    Application
    • 49
    • 21
    • 18
    • 14
    • 13
    • 8
    • 6
    • 5
    • 3
    • 1
    Western Blotting (WB), Immunofluorescence (IF)
    Cross-Reactivity
    Human, Dog (Canine), Rat (Rattus)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Mouse Hip1R aa. 560-772
    Clone
    44-Hip1R
    Isotype
    IgG1
    Top Product
    Discover our top product HIP1R Primary Antibody
  • Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20°C.
  • Itoh, Koshiba, Kigawa, Kikuchi, Yokoyama, Takenawa: "Role of the ENTH domain in phosphatidylinositol-4,5-bisphosphate binding and endocytosis." in: Science (New York, N.Y.), Vol. 291, Issue 5506, pp. 1047-51, (2001) (PubMed).

    Engqvist-Goldstein, Kessels, Chopra, Hayden, Drubin: "An actin-binding protein of the Sla2/Huntingtin interacting protein 1 family is a novel component of clathrin-coated pits and vesicles." in: The Journal of cell biology, Vol. 147, Issue 7, pp. 1503-18, (2000) (PubMed).

    Seki, Muramatsu, Sugano, Suzuki, Nakagawara, Ohhira, Hayashi, Hori, Saito: "Cloning, expression analysis, and chromosomal localization of HIP1R, an isolog of huntingtin interacting protein (HIP1)." in: Journal of human genetics, Vol. 43, Issue 4, pp. 268-71, (1999) (PubMed).

  • Target
    HIP1R (Huntingtin Interacting Protein 1 Related (HIP1R))
    Alternative Name
    Hip1R (HIP1R Products)
    Synonyms
    HIP12 antibody, HIP3 antibody, ILWEQ antibody, AA410023 antibody, mKIAA0655 antibody, HIP1R antibody, hip1r antibody, hip3 antibody, hip12 antibody, ilweq antibody, DKFZp469N2015 antibody, hip1 antibody, MGC78813 antibody, huntingtin interacting protein 1 related antibody, huntingtin interacting protein 1 related b antibody, huntingtin interacting protein 1 related L homeolog antibody, HIP1R antibody, Hip1r antibody, hip1rb antibody, hip1r antibody, hip1r.L antibody
    Background
    Clathrin is the major protein component in the coat formed around pits and vesicles involved in receptor-mediated endocytosis. Clathrin forms a non-covalently bound triskelion structure composed of three heavy chains (192kDa each) and three light chains (23-25kDa each). A variety of proteins facilitate receptor-mediated endocytosis through association with clathrin-coated vesicles. Huntingtin interacting protein 1 (Hip1) is an actin-binding protein that interacts with Huntingtin protein, and has been implicated in vesicular transport defects found in Huntingtin's disease. Hip1 related protein (Hip1R) is another actin binding protein that contains an epsin NH2-terminal homology (ENTH)domain, three coiled-coil regions, a leucine zipper, and a talin-like actin binding domain. Hip1R mRNA is widely expressed, and Hip1R protein is enriched in the cell cortex and perinuclear region. The ENTH domain of Hip1R is required for binding to phosphatidylinositol-4,5-bisphosphate, and this complex is essential for clathrin-mediated endocytosis. In addition, Hip1R colocalizes with clathrin, AP-2 and endocytosed transferrin. Thus, Hip1R may facilitate interactions between clathrin-coated pits and actin during endocytosis.
    Molecular Weight
    120 kDa
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