F11R antibody (AA 126-237)

Catalog No. ABIN968747

Product Details anti-F11R Antibody

Target: F11R (F11 Receptor (F11R))

Binding Specificity: AA 126-237

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This F11R antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human JAM aa. 126-237

Clone: 43-JAM

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-F11R antibody (ABIN968747)

Gupta, Pillarisetti, Ohlstein: "Platelet agonist F11 receptor is a member of the immunoglobulin superfamily and identical with junctional adhesion molecule (JAM): regulation of expression in human endothelial cells and macrophages." in: IUBMB life, Vol. 50, Issue 1, pp. 51-6, (2001) (PubMed).

Ozaki, Ishii, Arai, Horiuchi, Kawamoto, Suzuki, Kita: "Junctional adhesion molecule (JAM) is phosphorylated by protein kinase C upon platelet activation." in: Biochemical and biophysical research communications, Vol. 276, Issue 3, pp. 873-8, (2000) (PubMed).

Ozaki, Ishii, Horiuchi, Arai, Kawamoto, Okawa, Iwamatsu, Kita: "Cutting edge: combined treatment of TNF-alpha and IFN-gamma causes redistribution of junctional adhesion molecule in human endothelial cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 2, pp. 553-7, (1999) (PubMed).

Martìn-Padura, Lostaglio, Schneemann, Williams, Romano, Fruscella, Panzeri, Stoppacciaro, Ruco, Villa, Simmons, Dejana: "Junctional adhesion molecule, a novel member of the immunoglobulin superfamily that distributes at intercellular junctions and modulates monocyte transmigration." in: The Journal of cell biology, Vol. 142, Issue 1, pp. 117-27, (1998) (PubMed).

Target Details for F11R

Target: F11R (F11 Receptor (F11R))

Alternative Name: JAM-1 (F11R Products)

Synonyms: CD321 antibody, JAM antibody, JAM1 antibody, JAMA antibody, JCAM antibody, KAT antibody, PAM-1 antibody, 9130004G24 antibody, AA638916 antibody, ESTM33 antibody, JAM-1 antibody, JAM-A antibody, Jcam antibody, Jcam1 antibody, Ly106 antibody, Jam1 antibody, F11 receptor antibody, F11R antibody, F11r antibody

Background: Tight junctions (zonulae occludens) are critical to the maintenance of cell polarity and intercellular barriers. Protein components of the tight junctions include actin filaments, symplekin, occludin, ZO-1, ZO-2, and ZO-3. Junctional adhesion molecules, JAM-1, -2, and -3, are a new subfamily in the immunoglobulin (Ig) superfamily that colocalize with these components of tight junctions. JAM-1 was identified through a screen of antibodies that recognizes antigens located at cell-cell contacts. The structure of JAM-1 includes two V-type Ig domains in the extracellular region, two N-glycosylation sites, and a small C-terminal cytoplasmic region. JAM-1 protein is expressed at cell-cell junctions in endothelial and epithelial cells, and antibodies to JAM-1 inhibit spontaneous and chemokine-induced monocyte transmigration through an endothelial cell monolayer. In addition, TNFalpha and IFN-gamma cause redistribution of JAM-1 from intercellular junctions to the cytoplasm. JAM-1 also is the receptor for the platelet membrane protein, F11 antigen, and activation of platelets leads to PKC phosphorylation of JAM-1. Thus, JAM-1 is a junctional adhesion molecule that functions in endothelial, epithelial, and platelet adhesion.

Molecular Weight: 38 kDa

Pathways: Cell-Cell Junction Organization