Calreticulin antibody (AA 270-390)

Catalog No. ABIN968752

Product Details anti-Calreticulin Antibody

Target: Calreticulin (CALR)

Binding Specificity: AA 270-390

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calreticulin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Human, Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Calreticulin aa. 270-390

Clone: 16-Calreticulin

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-Calreticulin antibody (ABIN968752)

Ito, Seyama, Kubota: "Calreticulin is directly involved in anti-alpha3 integrin antibody-mediated secretion and activation of matrix metalloprotease-2." in: Biochemical and biophysical research communications, Vol. 283, Issue 2, pp. 297-302, (2001) (PubMed).

Saito, Ihara, Leach, Cohen-Doyle, Williams: "Calreticulin functions in vitro as a molecular chaperone for both glycosylated and non-glycosylated proteins." in: The EMBO journal, Vol. 18, Issue 23, pp. 6718-29, (2000) (PubMed).

Nair, Wearsch, Mitchell, Wassenberg, Gilboa, Nicchitta: "Calreticulin displays in vivo peptide-binding activity and can elicit CTL responses against bound peptides." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 11, pp. 6426-32, (1999) (PubMed).

Mazzarella, Gold, Cunningham, Green: "Determination of the sequence of an expressible cDNA clone encoding ERp60/calregulin by the use of a novel nested set method." in: Gene, Vol. 120, Issue 2, pp. 217-25, (1992) (PubMed).

Target Details for Calreticulin

Target: Calreticulin (CALR)

Alternative Name: Calreticulin (CALR Products)

Synonyms: CRT antibody, RO antibody, SSA antibody, cC1qR antibody, crc antibody, crt antibody, Calregulin antibody, CG9429 antibody, Clr antibody, Dmel\\CG9429 antibody, anon-EST:Liang-2.11 antibody, anon-EST:Liang-2.17 antibody, anon-EST:fe3D7 antibody, clone 2.11 antibody, clone 2.17 antibody, eth antibody, potp antibody, cc1qr antibody, ssa antibody, NV19007 antibody, CRP55 antibody, ERp60 antibody, HACBP antibody, CHOT1 antibody, CHT1 antibody, CT1 antibody, AA589632 antibody, CRTR antibody, Creat antibody, CCDS1 antibody, calrl2 antibody, fc66d10 antibody, wu:fc66d10 antibody, wu:fj50c03 antibody, zgc:66153 antibody, calreticulin antibody, calreticulin L homeolog antibody, Calreticulin antibody, putative calreticulin antibody, solute carrier family 6 member 8 antibody, solute carrier family 6 (neurotransmitter transporter, creatine), member 8 antibody, CALR antibody, calr.L antibody, Crt antibody, crt antibody, CRT antibody, Calr antibody, calr antibody, LMJF_31_2600 antibody, Crc antibody, Slc6a8 antibody, SLC6A8 antibody, LOC8269812 antibody

Background: Proteins undergo numerous modifications, including folding, translocation, and degradation. During such modifications, polypeptides are rarely in a native, stable state. Molecular chaperones are a diverse group of proteins that modulate polypeptide stability through ATP-dependent folding. Many chaperone proteins are found in the endoplasmic reticulum (ER). Calreticulin is a luminal ER protein that is 39% homologous to the ER chaperone protein, calnexin. Calreticulin contains a C-terminal KDEL ER retention signal, and can bind Ca2+, Zn2+, ATP, and the proteins, ERp57 and protein disulfide isomerase. The molecular chaperone activities of calreticulin may include folding of both Asn-linked glycoproteins and non-glycosylated proteins. In addition, calreticulin is a component of MHC I/transporter associated with Ag presentation (TAP) complex where it may function in peptide assembly onto nascent class I molecules. Calreticulin may also function in integrin signaling, since it binds a3-integrin subunits and regulates integrin-mediated metalloprotease secretion. Thus, calreticulin may be involved in Ca2+ storage, cell adhesion, and protein folding. This antibody is routinely tested by western blot analysis.

Molecular Weight: 60 kDa

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response