Leucine Rich Repeat (In FLII) Interacting Protein 1 (LRRFIP1) (AA 210-415) antibody

Antigen: Leucine Rich Repeat (In FLII) Interacting Protein 1 (LRRFIP1)

Synonyms: Fliiap1, AU024550, LRRFIP1, MGC151609

Binding Site: AA 210-415

Clonality: Monoclonal (32)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968764

Additional Information

Alternative name: GCF2

Immunogen: Human GCF2

Format: Liquid

Isotype: IgG1

Clone: 32

Description: Regulation of transcription is mediated by transcription factors that bind regulatory elements in gene promotors and enhancers, and alter the rate of transcription. Transcriptional repressors reduce transcription by their intrinsic repressor domain activity, or by binding to sites in the regulatory domain where a co-repressor can bind. GC-binding factors (GCF and GCF2) are transcriptional repressors that bind promoters with GC-rich sequences. GCF represses transcription of EGF, TGFalpha, and insulin-like growth factor II recepetors. GCF2 has some homology with GCF, and has also been identified as TRIP and LRRFIP1. The sequence of GCF2 includes an RNA binding domain (RBD), and N-glycosylation site, and a nuclear localization signal (NLS). GCF2 is widely expressed in many tissues and cells. GCF2 binds the EGFR and PDGFR promoters, and represses both EGFR and PDGFR transcription. In addition, GCF2 interacts with the leucine-rich repeat of flightless I, a protein that binds actin and contains a gelsolin-like domain. Thus, GCF2 may have functions in both RNA-cytoskeleton interactions, as well as roles in transcriptional repression. This antibody is routinely tested by western blot analysis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 160 kDa

Comments:

Related Products: ABIN968533, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of GCF2 on a A431 lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- human GCF2 antibody.

Immunofluoresence staining of A431 cells.

Publications

Product: Wilson, Brown, Kingsman et al.: "TRIP: a novel double stranded RNA binding protein which interacts with the leucine rich repeat of flightless I." in: Nucleic acids research, Vol. 26, Issue 15, pp. 3460-7, 1998 (PubMed).

Reed, Yamazaki, Kaufman et al.: "Molecular cloning and characterization of a transcription regulator with homology to GC-binding factor." in: The Journal of biological chemistry, Vol. 273, Issue 34, pp. 21594-602, 1998 (PubMed).

Khachigian, Santiago, Rafty et al.: "GC factor 2 represses platelet-derived growth factor A-chain gene transcription and is itself induced by arterial injury." in: Circulation research, Vol. 84, Issue 11, pp. 1258-67, 1999 (PubMed).