PDZ Binding Kinase (PBK) (AA 191-322) antibody
|Synonyms||SPK, CT84, TOPK, Nori-3, FLJ14385, AW538537, D14Ertd732e, 2810434B10Rik, topk, zgc:92050, PBK, MGC165897|
Alternatives Western Blotting (WB)
|3 references available|
|Price||Product not available in this region.|
|Cross-Reactivity||Mouse (Murine), Rat (Rattus)|
|Description||Mitogen activated protein (MAP) kinase signal transduction pathways mediate the effects of various extracellular stimuli on biological processes such as proliferation, differentiation, and death. The p38 MAP kinases are activated by dual phosphorylation on Thr and Tyr within the motif Thr-Gly-Tyr located in kinase subdomain VIII. Activation of p38 MAPK is mediated specifically by the MAP kinase kinases, MKK3 and MKK6. PBK is a PDZ-binding Ser-Thr kinase related to the MKK3/6 MAPKK family. PBK was also identified as T-LAK cell- originated protein kinase (TOPK) and spermatogenesis-related protein kinase (SPK). PBK contains the conserved dual specficity active site sequence (D-X-K-X-X-N) at amino acids 174 to 179, and a C-terminal ETDV motif that binds PDZ domains. The mRNA expression of PBK is reportedly highest in placenta, and lower in heart and pancreas. In mitotic cells, PBK is phosphorylated and enzymatically active, and in vitro, PBK is phosphorylated by Cdc2/cyclin B. PBK can bind to the PDZ domain-containing protein, Dlg, and can phosphorylate p38 MAPK. Thus, PBK is a MAPKK family member that may be important for regulation of PDZ domain proteins during mitosis. This antibody is routinely tested by western blot analysis.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Molecular Weight||41 kDa|
Related Products: ABIN968537, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20° C.|
|Research Area||Cancer, Cell Cycle, DNA/RNA|
|Restrictions||For Research Use only|
|Western blot analysis of PBK on a Jurkat cell lysate (Human T-cell leukemia, ATCC TIB-152). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti-PBK antibody.|
Gaudet, Branton, Lue: "Characterization of PDZ-binding kinase, a mitotic kinase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 10, pp. 5167-72, 2000 (PubMed).
Abe, Matsumoto, Kito et al.: "Cloning and expression of a novel MAPKK-like protein kinase, lymphokine-activated killer T-cell-originated protein kinase, specifically expressed in the testis and activated lymphoid cells." in: The Journal of biological chemistry, Vol. 275, Issue 28, pp. 21525-31, 2000 (PubMed).
Zhao, Dai, Zhao et al.: "PDZ-binding kinase participates in spermatogenesis." in: The international journal of biochemistry & cell biology, Vol. 33, Issue 6, pp. 631-6, 2001 (PubMed).
|Hosts||Rabbit (60), Mouse (12)|
|Reactivities||Human (71), Mouse (Murine) (28), Rat (Rattus) (28), Cow (Bovine) (20), Dog (Canine) (20), Pig (Porcine) (19), Cat (Feline) (1), Chicken (1), Xenopus laevis (1), Zebrafish (1)|
|Applications||ELISA (46), Western Blotting (WB) (43), Immunofluorescence (IF) (35), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (22), Immunohistochemistry (IHC) (12), Flow Cytometry (FACS) (4), Immunoprecipitation (IP) (4), Immunocytochemistry (ICC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), PE,Cy5.5 (2), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)|
|Epitopes||pThr9 (8), C-Term (7), N-Term (6), pTyr74 (3), Tyr74 (2), C-Term,Cys300 (1), Met11 (1), Met12 (1), N-Term,Asn6 (1), pThr163 (1)|