VAMP (Vesicle-Associated Membrane Protein)-Associated Protein A, 33kDa (VAPA) (AA 119-226) antibody

Antigen: VAMP (Vesicle-Associated Membrane Protein)-Associated Protein A, 33kDa (VAPA)

Binding Site: AA 119-226

Clonality: Monoclonal (8)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968776

Additional Information

Alternative name: VAP33

Immunogen: Mouse VAP33

Cross-Reactivity: Human, Dog (Canine), Rat (Rattus)

Format: Liquid

Isotype: IgM

Clone: 8

Description: In eukaryotic cells, trafficking of membrane and secretory proteins requires an elaborate system of organelles, vesicles, and cytoskeletal structures. Proteins important for protein trafficking usually interact with one or all of these cellular structures. VAP33 (VAP-A) was identified through its ability to bind the synaptic vesicle protein synaptobrevin/VAMP-1. The structure of VAP33 includes an N-terminal domain similar to the major sperm protein from Ascaris lubricoides, a central coiled-coil domain, and a C-terminal transmembrane region. VAP33 mRNA is expressed at high levels in testis, but is also found in most other tissues. In rat neurons, VAP33 localizes to the ER and microtubules, while in many cells and tissues, VAP33 co-localizes to tight junctions along with occludin. Interestingly, 83% of VAP33 fractionates with occludin and DPPIV in the plasma membrane fraction, while only 14% fractionates in the vesicular pool. In L6 skeletal myoblasts, VAP33 colocalizes with VAMP-2, and overexpression of VAP33 attenuates insulin-dependent incorporation of GLUT4 into the plasma membrane. This effect can be suppressed by overexpression of VAMP-2. Thus, VAP33 may be involved in the trafficking of plasma membrane proteins to specific sites within the cell.
Synonyms: VAP-A

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 33 kDa

Comments:

Related Products: ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of VAP33 on a mouse testis lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-VAP33 antibody.

Immunofluorescence staining of SK-N-MC cells (Human neuroblastoma, ATCC HTB-10).

Publications

Product: Nishimura, Hayashi, Inada et al.: "Molecular cloning and characterization of mammalian homologues of vesicle-associated membrane protein-associated (VAMP-associated) proteins." in: Biochemical and biophysical research communications, Vol. 254, Issue 1, pp. 21-6, 1999 (PubMed).

Lapierre, Tuma, Navarre et al.: "VAP-33 localizes to both an intracellular vesicle population and with occludin at the tight junction." in: Journal of cell science, Vol. 112 ( Pt 21), pp. 3723-32, 1999 (PubMed).

Skehel, Fabian-Fine, Kandel: "Mouse VAP33 is associated with the endoplasmic reticulum and microtubules." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 3, pp. 1101-6, 2000 (PubMed).

Foster, Weir, Lim et al.: "A functional role for VAP-33 in insulin-stimulated GLUT4 traffic." in: Traffic (Copenhagen, Denmark), Vol. 1, Issue 6, pp. 512-21, 2001 (PubMed).