Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 125-239), (pSer463), (pSer465), (pSer467) antibody

Details for Product No. ABIN968785
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Antigen
Synonyms Smac, DFNA64, SMAC, DBO, XSmac, smac, smac3, diablo-s
Epitope
AA 125-239, pSer463, pSer465, pSer467
(27), (8), (7), (4), (3), (3), (3), (2), (2), (2), (1)
Reactivity
Human
(122), (54), (47), (14), (14), (13), (13), (2), (1)
Host
Mouse
(111), (20), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(122), (42), (39), (35), (15), (10), (6), (5), (4), (3), (3), (3), (2), (2), (1), (1)
Pubmed 4 references available
Catalog no. ABIN968785
Quantity 50 µg
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Immunogen Human Smac/DIABLO aa. 125-239
Clone 56
Isotype IgG2b
Cross-Reactivity Rat (Rattus), Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name Smac/DIABLO
Background Apoptosis is a genetically programmed, selective process of cell death that occurs during normal cell differentiation and development of multicellular organisms. Inhibitors of apoptosis proteins (IAPs), hILP/XIAP, c-IAP1, c-IAP2, and survivin, suppress apoptosis by preventing procaspase activation and inhibiting mature caspase activity. Smac/DIABLO (second mitochondria-derived activator of caspases) eliminates the inhibitory effect of many IAPs, and has been shown to interact with hILP, c-IAP1, c-IAP2, and survivin. The interaction with hILP occurs through Smac/DIABLO binding to the second and third BIR domains of hILP. Smac/DIABLO protein contains an N-terminal mitochondria targeting sequence (MTS) and a procaspase-3 activation domain. The N-terminal MTS is cleaved after import into the mitochondria, and the activity of the mature Smac/DIABLO is dependent on homodimerization and release from the mitochondria. Overexpression of Smac/DIABLO in HeLa cells increases the sensitivity to UV-mediated apoptosis. Thus, Smac/DIABLO acts as an apoptotic activator through its binding to IAPs and activation of caspases.
Molecular Weight 22 kDa
Research Area Apoptosis/Necrosis
Comment

Related Products: ABIN968535, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 125-239), (pSer463), (pSer465), (pSer467) antibody Western blot analysis of Smac/DIABLO on a HeLa cell lysate (Human cervical epitheloid carcinoma, ATCC CCL-2). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-Smac/DIABLO antibody.
Product cited in: Ikuta, Weissman: "Evidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 4, pp. 1502-6, 1992 (PubMed).

Du, Fang, Li et al.: "Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition." in: Cell, Vol. 102, Issue 1, pp. 33-42, 2000 (PubMed).

Chai, Du, Wu et al.: "Structural and biochemical basis of apoptotic activation by Smac/DIABLO." in: Nature, Vol. 406, Issue 6798, pp. 855-62, 2000 (PubMed).

Kim, Broxmeyer: "Therapeutic potential of 4-1BB (CD137) as a regulator for effector CD8(+) T cells." in: Journal of hematotherapy & stem cell research, Vol. 10, Issue 4, pp. 441-9, 2001 (PubMed).

Hosts (111), (20), (5)
Reactivities (122), (54), (47), (14), (14), (13), (13), (2), (1)
Applications (122), (42), (39), (35), (15), (10), (6), (5), (4), (3), (3), (3), (2), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (27), (8), (7), (4), (3), (3), (3), (2), (2), (2), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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