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ARHGAP4 antibody (AA 843-955)

ARHGAP4 Reactivity: Mouse, Rat WB, IF Host: Mouse Monoclonal 15-p115 unconjugated
Catalog No. ABIN968790
  • Target See all ARHGAP4 Antibodies
    ARHGAP4 (rho GTPase Activating Protein 4 (ARHGAP4))
    Binding Specificity
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 843-955
    Reactivity
    • 20
    • 2
    • 2
    • 1
    Mouse, Rat
    Host
    • 18
    • 3
    Mouse
    Clonality
    • 19
    • 2
    Monoclonal
    Conjugate
    • 14
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ARHGAP4 antibody is un-conjugated
    Application
    • 13
    • 4
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF)
    Cross-Reactivity
    Mouse (Murine)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Rat p115 aa. 843-955
    Clone
    15-p115
    Isotype
    IgG1
    Top Product
    Discover our top product ARHGAP4 Primary Antibody
  • Comment

    Related Products: ABIN968545, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20° C.
  • Mary, Charrasse, Meriane, Comunale, Travo, Blangy, Gauthier-Rouvière: "Biogenesis of N-cadherin-dependent cell-cell contacts in living fibroblasts is a microtubule-dependent kinesin-driven mechanism." in: Molecular biology of the cell, Vol. 13, Issue 1, pp. 285-301, (2002) (PubMed).

    Barroso, Nelson, Sztul: "Transcytosis-associated protein (TAP)/p115 is a general fusion factor required for binding of vesicles to acceptor membranes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 2, pp. 527-31, (1995) (PubMed).

    Waters, Clary, Rothman: "A novel 115-kD peripheral membrane protein is required for intercisternal transport in the Golgi stack." in: The Journal of cell biology, Vol. 118, Issue 5, pp. 1015-26, (1992) (PubMed).

  • Target
    ARHGAP4 (rho GTPase Activating Protein 4 (ARHGAP4))
    Alternative Name
    p115 (ARHGAP4 Products)
    Synonyms
    zgc:63672 antibody, ARHGAP4 antibody, C1 antibody, RGC1 antibody, RhoGAP4 antibody, SrGAP4 antibody, p115 antibody, A130009C12Rik antibody, A530015A20Rik antibody, Rgc1 antibody, c1 antibody, mKIAA0131 antibody, Rho GTPase activating protein 4a antibody, Rho GTPase activating protein 4 antibody, arhgap4a antibody, ARHGAP4 antibody, arhgap4 antibody, Arhgap4 antibody
    Background
    Maturation and post translational modification of proteins occurs after their biosynthesis at the endoplasmic reticulum and their transport through the Golgi apparatus. The process involves the transport of vesicles carrying the proteins through a vectorial process of vesicle budding and fusion from the cis-compartment to the medial-compartment and the trans-compartment of the Golgi apparatus. p115 is a 959 amino acid protein located at the Golgi apparatus that, with the NEM-sensitive fusion protein and the soluble NSF attachment protein (SNAP), is required for vesicle transport from the cis-compartment to the medial-compartment. p115 protein is related to the yeast Uso1p essential for the vesicular transport from the endoplasmic reticulum to the Golgi. Native p115 appears to be a homo-oligomer, with two globular heads and a tail that resemble the overall structure of myosin. p115 is extracted from the Golgi apparatus with high salt or high pH, indicative of a membrane associated protein. p115 interacts with the golgi matrix protein GM130 but this interaction is disrupted by the Golgi fragmentation during mitosis and the phosphorylation of GM130. This antibody is routinely tested by western blot analysis.
    Molecular Weight
    115 kDa
    Pathways
    Neurotrophin Signaling Pathway, Regulation of Cell Size
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