GAP43 antibody (AA 1-144)

Catalog No. ABIN968792

Product Details anti-GAP43 Antibody

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Binding Specificity: AA 1-144

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAP43 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Rat (Rattus), Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human GAP-43/Neuromodulin aa. 1-144

Clone: 31-GAP

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-GAP43 antibody (ABIN968792)

Chapman, Estep, Storm: "Palmitylation of neuromodulin (GAP-43) is not required for phosphorylation by protein kinase C." in: The Journal of biological chemistry, Vol. 267, Issue 35, pp. 25233-8, (1993) (PubMed).

Meiri, Bickerstaff, Schwob: "Monoclonal antibodies show that kinase C phosphorylation of GAP-43 during axonogenesis is both spatially and temporally restricted in vivo." in: The Journal of cell biology, Vol. 112, Issue 5, pp. 991-1005, (1991) (PubMed).

Strittmatter, Valenzuela, Kennedy, Neer, Fishman: "G0 is a major growth cone protein subject to regulation by GAP-43." in: Nature, Vol. 344, Issue 6269, pp. 836-41, (1990) (PubMed).

Cimler, Andreasen, Andreasen, Storm: "P-57 is a neural specific calmodulin-binding protein." in: The Journal of biological chemistry, Vol. 260, Issue 19, pp. 10784-8, (1985) (PubMed).

Target Details for GAP43

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Alternative Name: GAP-43/Neuromodulin (GAP43 Products)

Synonyms: B-50 antibody, PP46 antibody, Basp2 antibody, GAP-43 antibody, b-50 antibody, gap-43 antibody, gap43b antibody, pp46 antibody, cb310 antibody, gap antibody, gap43 antibody, gap43a antibody, growth associated protein 43 antibody, growth associated protein 43 L homeolog antibody, growth associated protein 43 S homeolog antibody, GAP43 antibody, Gap43 antibody, gap43.L antibody, gap43 antibody, gap43.S antibody

Background: Neuromodulin (GAP-43, B-50, F-1) is a neural-specific protein that represents about one percent of the total membrane protein in growth cones from developing rat brain. Growth cones are located at the distal tip of neuronal processes and are critical for transduction of extracellular signals into directed growth. Neuromodulin is attached to the membranes of the neuronal growth cones during development. In the adult, it is attached to the presynaptic membranes in discrete subsets of synapses. Neuromodulin can bind to calmodulin, as well as inhibit phosphatidylinositol phosphate kinase (PIP kinase) activity. It is also a prominent substrate of Protein Kinase C (PKC) and may mediate some of the effects of PKC on growth cone function. As may be expected, expression and phosphorylation of neuromodulin are closely associated with axonal outgrowth and repair. Phosphorylation of neuromodulin by PKC has been correlated with the persistence of long term potentiation and the translocation of PKC to synaptosomal membranes. This PKC-mediated phosphorylation does not appear to require neuromodulin membrane association. It has also been demonstrated that the GTP-binding protein, G[o], is a main component of growth cone membranes. The binding of GTP--S to G[o] is stimulated by neuromodulin and this activity resides within the amino-terminal region of the protein. This region of neuromodulin shares sequence homology with the cytoplasmic tails of G-linked transmembrane receptors, suggesting the presence of one binding site on G[o] for both neuromodulin and transmembrane receptors. This antibody is routinely tested by western blot analysis.

Molecular Weight: 43 kDa