TOMM20 antibody (AA 47-145)

Catalog No. ABIN968798

Product Details anti-TOMM20 Antibody

Target: TOMM20 (Mitochondrial Import Receptor Subunit TOM20 homolog (TOMM20))

Binding Specificity: AA 47-145

Reactivity: Human, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This TOMM20 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Dog (Canine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Tom20 aa. 47-145

Clone: 29-Tom20

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-TOMM20 antibody (ABIN968798)

Krimmer, Rapaport, Ryan, Meisinger, Kassenbrock, Blachly-Dyson, Forte, Douglas, Neupert, Nargang, Pfanner: "Biogenesis of porin of the outer mitochondrial membrane involves an import pathway via receptors and the general import pore of the TOM complex." in: The Journal of cell biology, Vol. 152, Issue 2, pp. 289-300, (2001) (PubMed).

Abe, Shodai, Muto, Mihara, Torii, Nishikawa, Endo, Kohda: "Structural basis of presequence recognition by the mitochondrial protein import receptor Tom20." in: Cell, Vol. 100, Issue 5, pp. 551-60, (2000) (PubMed).

Hernández, Giner, Hernández-Yago: "Gene structure of the human mitochondrial outer membrane receptor Tom20 and evolutionary study of its family of processed pseudogenes." in: Gene, Vol. 239, Issue 2, pp. 283-91, (1999) (PubMed).

Target Details for TOMM20

Target: TOMM20 (Mitochondrial Import Receptor Subunit TOM20 homolog (TOMM20))

Alternative Name: Tom20 (TOMM20 Products)

Synonyms: MAS20 antibody, MOM19 antibody, TOM20 antibody, 1810060K07Rik antibody, BB284719 antibody, Gm19268 antibody, mKIAA0016 antibody, tomm20 antibody, wu:fk47b11 antibody, zgc:73165 antibody, mas20 antibody, mom19 antibody, tom20 antibody, zgc:92628 antibody, translocase of outer mitochondrial membrane 20 antibody, translocase of outer mitochondrial membrane 20 homolog (yeast) antibody, Mitochondrial import receptor subunit TOM20 homolog antibody, translocase of outer mitochondrial membrane 20 homolog L homeolog antibody, translocase of outer mitochondrial membrane 20b antibody, TOMM20 antibody, Tomm20 antibody, tomm20a antibody, tomm-20 antibody, tomm20.L antibody, tomm20b antibody

Background: Many mitochondrial proteins are encoded by nuclear genes and synthesized in the cytosol as pre-proteins. These pre-proteins are targeted to the mitochondria where they are translocated across the outer and inner membranes of the mitochondria. At the outer membrane of the mitochondria, this process requires a multiprotein complex called the Translocase of the Outer Membrane of the mitochondria (TOM). The TOM complex includes receptor components, Tom20, 22, 37, and 70, as well as a multisubunit general import pore complex of 400 kDa. Tom20 contains a hydrophobic N-terminal region that anchors it to the outer membrane of the mitochondria, and a cytosolic region that contains a domain that facilitates binding to the basic amphiphilic targeting sequences of pre-proteins and a tetratricopeptide repeat (TPR) motif involved in interactions with Tom70. In yeast, Tom20 and Tom22 mediate the import of all pre-proteins known to use the general import machinery of the mitochondria. Thus, Tom20 is an important receptor for many mitochondrial pre-proteins that use the outer mitochondrial translocation complex.

Molecular Weight: 18 kDa

Pathways: Unfolded Protein Response