Tyrosine Hydroxylase antibody (AA 18-133)

Catalog No. ABIN968808

Product Details anti-Tyrosine Hydroxylase Antibody

Target: Tyrosine Hydroxylase (TH)

Binding Specificity: AA 18-133

Reactivity: Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Tyrosine Hydroxylase antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat Tyrosine Hydroxylase aa. 18-133

Clone: 45-Tyrosine Hydroxylase

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389, ABIN968545

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-Tyrosine Hydroxylase antibody (ABIN968808)

Blanchard-Fillion, Souza, Friel, Jiang, Vrana, Sharov, Barrón, Schöneich, Quijano, Alvarez, Radi, Przedborski, Fernando, Horwitz, Ischiropoulos: "Nitration and inactivation of tyrosine hydroxylase by peroxynitrite." in: The Journal of biological chemistry, Vol. 276, Issue 49, pp. 46017-23, (2001) (PubMed).

Salvatore, Waymire, Haycock: "Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ." in: Journal of neurochemistry, Vol. 79, Issue 2, pp. 349-60, (2001) (PubMed).

Target Details for Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Alternative Name: Tyrosine Hydroxylase (TH Products)

Synonyms: DYT14 antibody, DYT5b antibody, TYH antibody, The antibody, CG10118 antibody, DH65B antibody, DTH antibody, Dmel\\CG10118 antibody, Pale antibody, Ple antibody, TH antibody, Th antibody, VII antibody, dTH1 antibody, dTH65B antibody, pale/ple antibody, th antibody, dyt14 antibody, dyt5b antibody, tyh antibody, tyrosine hydroxylase antibody, pale antibody, tyrosine 3-monooxygenase antibody, tyrosine hydroxylase S homeolog antibody, TH antibody, Th antibody, ple antibody, th antibody, th.S antibody, LOC100008895 antibody

Background: Dopamine and its metabolic products norepinephrine and epinephrine are catecholamine neurotransmitters whose function is essential for the execution of normal neural processes in the CNS and PNS. Tyrosine hydroxylase (TH) is a non-heme iron, tetrahydrobiopterin-dependent enzyme that catalzyes the conversion of tyrosine to L-dihydroxyphenylalanine (L-DOPA). This is the rate-limiting step in the biosynthesis of catecholamines. Both the development of Parkinson’s disease and other neurodegenerative diseases result from loss of the ability to synthesize catecholamines. Decreases in the activity of TH have been implicated in these diseases. Nitration of TH at Tyrosine 423 has been associated with temporary loss of enzymatic activity, and TH nitration occurs in response to the Parkinsonian toxin MPTP, and following exposure to peroxynitrite. These findings implicate nitration as a potential mode of down-regulation of TH activity during neurodegenerative disease. Thus, TH is an essential enzyme for catecholamine synthesis, which is required for normal neuronal function.

Molecular Weight: 58 kDa

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour