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KIT antibody (Mast/stem Cell Growth Factor Receptor) (AA 1-115)

Details for Product anti-KIT Antibody No. ABIN968819, Supplier: Log in to see
Antigen
  • Bs
  • C-Kit
  • c-kit
  • c-KIT
  • CD117
  • Fdc
  • Gsfsco1
  • Gsfsco5
  • Gsfsow3
  • MGF
  • PBT
  • SCFR
  • SCO1
  • SCO5
  • SOW3
  • Ssm
  • Tr-kit
  • W
Alternatives
anti-Human KIT antibody for Immunofluorescence
Epitope
AA 1-115
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65
41
37
32
25
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19
15
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Reactivity
Human
883
426
138
22
20
17
15
6
2
Host
Mouse
633
294
234
8
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1
Clonality (Clone)
Monoclonal ()
Conjugate
This KIT antibody is un-conjugated
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Application
Western Blotting (WB)
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Supplier
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Immunogen Human c-Kit
Clone MOC-31
Isotype IgG2a, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name CD117 (KIT Antibody Abstract)
Background C-Kit, a type III tyrosine kinase receptor, binding of stem cell factor (SCF) is involved in the regulation of a wide range of tissues at various developmental stages. c-Kit is a 145 kD protein with an N-terminal domain containing an extracellular domain and a transmembrane domain (TMD) typical of a transmembrane receptor kinase and has been renamed as CD117 with the new nomenclature. SCF and c-kit play a major role in regulation of human haematopoiesis as evidenced by increases in c-kit expression when erythroid differentiation is inhibited with basic fibroblast growth factor. Activating mutations of c-kit are associated with sporadic adult human mastocytosis (SAHM), gastrointestinal stromal tumors (GIST), acute myeloid leukemia (AML), and germ cell tumors. Activation of STAT-3 may be a key requirement for c-kit tumorigenicity. Human germ cell proliferation and survival are highly dependent on c-kit expression and genetic mutations of c-kit result in infertility, disruption of spermatogenesis, and some testicular disorders. Blockage of SCF binding to c-kit via inhibitors of the ligand may be a viable clinical approach in management of GIST and possibly other diseases. Thus, c-kit plays a major role in reproductive development and in many human diseases, including various cancers.
Synonyms: c-Kit
Molecular Weight 145 kDa
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Western Blotting (WB) image for anti-KIT antibody (Mast/stem Cell Growth Factor Receptor) (AA 1-115) (ABIN968819) Western blot analysis of CD117 on HEL lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3:...
Product cited in: Burger, Lukey, Coetzee, Wilson: "Basic fibroblast growth factor modulates the expression of glycophorin A and c-kit and inhibits erythroid differentiation in K562 cells." in: Journal of cellular physiology, Vol. 190, Issue 1, pp. 83-91, 2002 (PubMed).

Ma, Zeng, Metcalfe, Akin, Dimitrijevic, Butterfield, McMahon, Longley et al.: "The c-KIT mutation causing human mastocytosis is resistant to STI571 and other KIT kinase inhibitors; kinases with enzymatic site mutations show different inhibitor sensitivity profiles than ..." in: Blood, Vol. 99, Issue 5, pp. 1741-4, 2002 (PubMed).

Ning, Li, McGuinness, Arceci: "STAT3 activation is required for Asp(816) mutant c-Kit induced tumorigenicity." in: Oncogene, Vol. 20, Issue 33, pp. 4528-36, 2001 (PubMed).

Robinson, Gaskell, Saunders, Anderson: "Germ cell specific expression of c-kit in the human fetal gonad." in: Molecular human reproduction, Vol. 7, Issue 9, pp. 845-52, 2001 (PubMed).

Yarden, Kuang, Yang-Feng, Coussens, Munemitsu, Dull, Chen, Schlessinger, Francke, Ullrich: "Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand." in: The EMBO journal, Vol. 6, Issue 11, pp. 3341-51, 1988 (PubMed).