TRAP1 antibody (AA 253-464)

Catalog No. ABIN968826

Product Details anti-TRAP1 Antibody

Target: TRAP1 (TNF Receptor-Associated Protein 1 (TRAP1))

Binding Specificity: AA 253-464

Reactivity: Human, Rat, Mouse, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Hsp75/TRAP1 aa. 253-464

Clone: 42-Hsp75

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-TRAP1 antibody (ABIN968826)

Felts, Owen, Nguyen, Trepel, Donner, Toft: "The hsp90-related protein TRAP1 is a mitochondrial protein with distinct functional properties." in: The Journal of biological chemistry, Vol. 275, Issue 5, pp. 3305-12, (2000) (PubMed).

Chen, Chen, Dai, Chen, Riley, Lee: "A new member of the hsp90 family of molecular chaperones interacts with the retinoblastoma protein during mitosis and after heat shock." in: Molecular and cellular biology, Vol. 16, Issue 9, pp. 4691-9, (1996) (PubMed).

Song, Dunbar, Zhang, Guo, Donner: "Identification of a protein with homology to hsp90 that binds the type 1 tumor necrosis factor receptor." in: The Journal of biological chemistry, Vol. 270, Issue 8, pp. 3574-81, (1995) (PubMed).

Target Details for TRAP1

Target: TRAP1 (TNF Receptor-Associated Protein 1 (TRAP1))

Alternative Name: Hsp75 (TRAP1 Products)

Synonyms: HSP 75 antibody, HSP75 antibody, HSP90L antibody, TRAP-1 antibody, 2410002K23Rik antibody, fc85a11 antibody, wu:fc85a11 antibody, TNF receptor associated protein 1 antibody, TNF receptor-associated protein 1 antibody, TRAP1 antibody, Trap1 antibody, trap1 antibody

Background: The hsp90 family of molecular chaperones is involved in a number of signal transduction pathways, including cell division, protein folding and transport. A new member of this family was recently identified as TRAP1 (Tumor necrosis factor receptor-associated protein) or hsp75. TRAP1 is a mitochondrial protein that is an ATPase and ATP-binding protein, rendering it similar to hsp90. There is a high degree of homology between TRAP1 and hsp90 in 6 domains, however one major difference is that a highly charged domain present in hsp90 is absent in TRAP1. TRAP1 has been shown to interact with the intracellular domain of the type 1 receptor for tumor necrosis factor (TNFR-1IC) and to the retinoblastoma protein (Rb). Signaling through TNFR-1 induces antiviral responses, fibroblast proliferation, cytotoxicity, etc. TRAP1 binds TNFR-1IC outside of the death domain leading to disruption in the signaling by TNFR-1. Rb has been shown to play a pivotal role in cellular differentiation and progression through the cell cycle. TRAP1 associates with Rb via an LxCxE motif, which is common among proteins that interact with Rb through SV40 T-antigen binding domain. Thus, TRAP1 is a new member of the hsp90 family that interacts with Rb and TNFR-1IC.
Synonyms: TRAP1

Molecular Weight: 75 kDa