Jagged 1 (JAG1) (AA 1015-1207) antibody

Antigen: Jagged 1 (JAG1)

Synonyms: AGS, AHD, AWS, HJ1, CD339, JAGL1, MGC104644, Htu, Ozz, Ser-1, slalom, Serrate-1, Headturner, JAG1

Binding Site: AA 1015-1207

Clonality: Monoclonal (21)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN968827

Additional Information

Alternative name: Jagged1

Immunogen: Human Jagged1

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine)

Format: Liquid

Isotype: IgG1

Clone: 21

Description: The Notch signaling pathway is integral to the appropriate differentiation of multiple cell types during development. Disruption of this gene in mice leads to widespread cell death and embryonic lethality before 11.5 days of gestation. Notch signaling has also been implicated in numerous human disease processes, such as leukemia and the congenital syndromes, Alagille and CADASIL. The Notch pathway has been shown to be important in human hematopoiesis as well. Notch is a transmembrane receptor activated by multiple ligands, including Jagged1, Jagged2, and Delta1. The sequence of Jagged1 is partially homologous to the Serrate and Delta Notch ligands of Drosophila and all three share a domain unique to Notch ligands, the DSL domain. Jagged1, a 130 kDa protein, is widely expressed in developing human tissues, brain, and hematopoietic cells. In the adult brain strongest expression is found in the hippocampal formation and the Purkinje cells of the cerebellum. In cell culture assays, Jagged1 binding of Notch prevented muscle cell differentiation. Therefore, Jagged1 activation of Notch signaling pathways is important for the differentiation and proliferation of various tissues.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 130 kDa

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of Jagged1 on EB-1 lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of Jagged1.

Publications

Product: Lindsell, Shawber, Boulter et al.: "Jagged: a mammalian ligand that activates Notch1." in: Cell, Vol. 80, Issue 6, pp. 909-17, 1995 (PubMed).

Jones, May, Healy et al.: "Stromal expression of Jagged 1 promotes colony formation by fetal hematopoietic progenitor cells." in: Blood, Vol. 92, Issue 5, pp. 1505-11, 1998 (PubMed).

Berezovska, Xia, Hyman: "Notch is expressed in adult brain, is coexpressed with presenilin-1, and is altered in Alzheimer disease." in: Journal of neuropathology and experimental neurology, Vol. 57, Issue 8, pp. 738-45, 1998 (PubMed).

Gray, Mann, Mitsiadis et al.: "Human ligands of the Notch receptor." in: The American journal of pathology, Vol. 154, Issue 3, pp. 785-94, 1999 (PubMed).