Immunofluorescent staining of U-2 OS (ATCC HTB-96) cells. Cells were seeded in a 96 well imaging plate at ~ 10 000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and the anti-CDC5L antibody. The second step reagent was FITC goat anti mouse Ig. Images were taken on a BD Pathway™ 855 bioimaging system using a 20x objective. This antibody also stained HeLa (ATCC CCL-2) cells and worked with both the Triton™ X-100 and alcohol perm protocols. This antibody is not recommended for staining A549 cells.