Catenin (Cadherin-Associated Protein), delta 1 (CTNND1) (pTyr96) antibody

Antigen: Catenin (Cadherin-Associated Protein), delta 1 (CTNND1)

Synonyms: CAS, p120, CTNND, P120CAS, P120CTN, KIAA0384, CTNND1, cas, Xp120, ctnnd, p120cas, p120ctn, p120(ctn), Xp120(ctn), p120-catenin, DKFZp469D181

Binding Site: pTyr96

Clonality: Monoclonal (25a)

Host: Mouse

Reactivity: Mouse (Murine)

Application: Western Blotting (WB)

Catalog no.: ABIN968862

Additional Information

Alternative name: p120 Catenin

Immunogen: Mouse p120 Catenin (pY96)

Cross-Reactivity: Human, Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 25a

Description: The membrane associated protein pp120 Src substrate (p120 Catenin, p120cas) was identified as a tyrosine kinase substrate that is phosphorylated in Src transformed cells or in response to growth factor stimulation. It shares structural similarity with the Drosophila Armadillo protein and the vertebrate beta-catenin and gamma-catenin proteins. This similarity is evidenced by its characteristic Arm domain that is composed of 42-amino acid motif repeats. In the cell, p120 Catenin is localized to the E-Cadherin/catenins cell adhesion complex. Like beta- and gamma-catenin, p120 Catenin directly associates with the cytoplasmic C-terminus of E-Cadherin via its Arm domain and may similarly interact with other Cadherins. It exists as four isoforms that range in size from 90-115kDa. Expression of these isoforms is heterogeneous in human carcinomas, suggesting that altered pp120 expression contributes to malignancy due to loss of functional cell adhesions. Multiple tyrosine residues (Y96, Y112, Y228, Y280, Y257, Y291, Y296, and Y302) in p120 Catenin are phosphorylated by Src and these phosphorylations may facilitate interaction with PTP1C/SHP-1 in response to EGF stimulation. Thus, p120 Catenin is an Arm domain protein that interacts with both cell adhesion molecules, such as cadherins and cell signaling molecules, such as PTP1C.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 120 kDa

Comments:

Related Products: ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Human endothelial cells were treated with 1 mM pervanadate for 15 minutes at 37°C (lane 2) or left untreated (lane 1). The top panel was probed with p120 Catenin (ABIN967770) and the bottom panel was probed with p120 Catenin (pY96) (ABIN968862).

Publications

Product: Reynolds, Daniel, McCrea et al.: "Identification of a new catenin: the tyrosine kinase substrate p120cas associates with E-cadherin complexes." in: Molecular and cellular biology, Vol. 14, Issue 12, pp. 8333-42, 1994 (PubMed).

Mariner, Anastasiadis, Keilhack et al.: "Identification of Src phosphorylation sites in the catenin p120ctn." in: The Journal of biological chemistry, Vol. 276, Issue 30, pp. 28006-13, 2001 (PubMed).