STAT3 antibody (pSer727)

Catalog No. ABIN968869

Product Details anti-STAT3 Antibody

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Binding Specificity: pSer727

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This STAT3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human Stat3

Clone: 49-p

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-STAT3 antibody (ABIN968869)

Kanai, Konda, Nakajima, Izumi, Kanda, Nanakin, Kubohara, Chiba: "Differentiation-inducing factor-1 (DIF-1) inhibits STAT3 activity involved in gastric cancer cell proliferation via MEK-ERK-dependent pathway." in: Oncogene, Vol. 22, Issue 4, pp. 548-54, (2003) (PubMed).

Schuringa, Dekker, Vellenga, Kruijer: "Sequential activation of Rac-1, SEK-1/MKK-4, and protein kinase Cdelta is required for interleukin-6-induced STAT3 Ser-727 phosphorylation and transactivation." in: The Journal of biological chemistry, Vol. 276, Issue 29, pp. 27709-15, (2001) (PubMed).

Smith, Crompton: "Expression of v-src in mammary epithelial cells induces transcription via STAT3." in: The Biochemical journal, Vol. 331 ( Pt 2), pp. 381-5, (1998) (PubMed).

Fu, Zhang: "Transcription factor p91 interacts with the epidermal growth factor receptor and mediates activation of the c-fos gene promoter." in: Cell, Vol. 74, Issue 6, pp. 1135-45, (1993) (PubMed).

Target Details for STAT3

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Alternative Name: Stat3 (STAT3 Products)

Synonyms: 1110034C02Rik antibody, AW109958 antibody, Aprf antibody, APRF antibody, HIES antibody, Xstat3 antibody, aprf antibody, hies antibody, stat3 antibody, wu:fc15d02 antibody, wu:fl59g06 antibody, z-Stat3 antibody, signal transducer and activator of transcription 3 antibody, signal transduction and activation of transcription 3 antibody, signal transducer and activator of transcription 3, gene 1 L homeolog antibody, signal transducer and activator of transcription 3 (acute-phase response factor) antibody, STAT3 antibody, stat3 antibody, Stat3 antibody, stat3.1.L antibody

Background: The Stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Seven mammalian Stat proteins have been identified: Stat1-4, Stat5a, 5b, and Stat6. Stat3 is a 92-kDa protein that is activated as a DNA binding protein through cytokines, such as IL-6, and growth factors, such as EGF. Stat3 is phophorylated at serine 727 (S727) via the MAPK pathway. The S727 residue is located at a conserved Pro-X-Ser-Pro sequence, which is recognized by the protein kinase ERK. Activation through the S727 residue is thought to lead to initiation of transcription. Upon activation, Stat3 dimerizes, translocates to the nucleus, and binds DNA response elements thereby regulating gene expression. It appears that Stat3 binds to DNA as a homodimer, but it is also capable of binding as a heterodimer with Stat1. In addition to serine phosphorylation, Stat3 is also phosphorylated at tyrosine 705 by JAK1 in response to cytokine stimulation. Stat3 is widely expressed and can bind to the sis-inducible element (SIE) site from the c-fos promoter. This site is similar to the GAS element that is present in IFN-g-induced genes. Thus, phosphorylation of S727 in Stat3 occurs in response to growth factors and cytokines, and is essential for normal transcription activity.
The 49/p-Stat3 monoclonal antibody recognizes the S727-phosphorylated form of Stat3 (isoform 1). The fluorochrome-conjugated formats have been evaluated using a human model system. However, the unconjugated form of this antibody (ABIN968868 or ABIN968869) is also effective for western blot analysis of human, mouse, and rat tissue.

Molecular Weight: 92 kDa

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor