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Phosphoserine antibody

Details for Product No. ABIN968872
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Antigen
Reactivity
Human, Rat (Rattus)
(62), (12), (8), (8), (7), (5), (1)
Host
Mouse
(40), (36)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(9), (8), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(70), (61), (43), (8), (3), (2), (2), (1), (1), (1), (1)
Pubmed 3 references available
Quantity 150 µg
Options
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Catalog No. ABIN968872
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Clone Hs-3
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Background Protein phosphorylation of serine and threonine residues is critical for the control of protein activity involved in various cellular events. An assortment of Ser/Thr kinases and phosphatases regulate serine and threonine phosphorylation in cell signaling pathways, such as growth factor, cytokine, chemokine, and stress response. Detection of serine and threonine phosphorylation can generally be monitored by antibodies that detect phosphoserine and phosphothreonine. Our clone 19 antibody specifically recognizes phosphoserine modifications on peptides in ELISA, while our clone 22a detects both phosphoserine and phosphothreonine modifications on peptides in ELISA. These antibodies are reported to be useful for Western blot, flow cytometry, and microscopy detection of phosphoserine and phosphothreonine levels.
Research Area Neurology, Metabolism, Amino Acids, Protein Modifications
Comment

Related Products: ABIN968873

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Phosphoserine antibody Immunofluorescence. Top row: anti-Phosphoserine. Bottom Row: anti-Phosphoserine/threonine (ABIN968873). First Panels: A431 untreated, Second Panels: A431 & CalyculinA/ Okadaic Acid (2hr).
Product cited in: Pawson, Scott: "Signaling through scaffold, anchoring, and adaptor proteins." in: Science (New York, N.Y.), Vol. 278, Issue 5346, pp. 2075-80, 1998 (PubMed).

Yan, Packer, Gooley et al.: "Protein phosphorylation: technologies for the identification of phosphoamino acids." in: Journal of chromatography. A, Vol. 808, Issue 1-2, pp. 23-41, 1998 (PubMed).

Brivanlou, Darnell: "Signal transduction and the control of gene expression." in: Science (New York, N.Y.), Vol. 295, Issue 5556, pp. 813-8, 2002 (PubMed).

Validation Images
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