Human, Rat (Rattus)
Alternatives Western Blotting (WB), Immunofluorescence (IF)
|3 references available|
|Quantity||150 µg (250 µg/ml) (Variants)|
|Price||Product not available in this region.|
|Description||Protein phosphorylation of serine and threonine residues is critical for the control of protein activity involved in various cellular events. An assortment of Ser/Thr kinases and phosphatases regulate serine and threonine phosphorylation in cell signaling pathways, such as growth factor, cytokine, chemokine, and stress response. Detection of serine and threonine phosphorylation can generally be monitored by antibodies that detect phosphoserine and phosphothreonine. Our clone 19 antibody specifically recognizes phosphoserine modifications on peptides in ELISA, while our clone 22a detects both phosphoserine and phosphothreonine modifications on peptides in ELISA. These antibodies are reported to be useful for Western blot, flow cytometry, and microscopy detection of phosphoserine and phosphothreonine levels.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Related Products: ABIN968873
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20° C.|
|Research Area||Neurology, Metabolism, Amino Acids, Protein Modifications|
|Restrictions||For Research Use only|
|Immunofluorescence. Top row: anti-Phosphoserine. Bottom Row: anti-Phosphoserine/threonine (ABIN968873). First Panels: A431 untreated, Second Panels: A431 & CalyculinA/ Okadaic Acid (2hr).|
Pawson, Scott: "Signaling through scaffold, anchoring, and adaptor proteins." in: Science (New York, N.Y.), Vol. 278, Issue 5346, pp. 2075-80, 1998 (PubMed).
Yan, Packer, Gooley et al.: "Protein phosphorylation: technologies for the identification of phosphoamino acids." in: Journal of chromatography. A, Vol. 808, Issue 1-2, pp. 23-41, 1998 (PubMed).
Brivanlou, Darnell: "Signal transduction and the control of gene expression." in: Science (New York, N.Y.), Vol. 295, Issue 5556, pp. 813-8, 2002 (PubMed).
|Hosts||Rabbit (34), Mouse (25)|
|Reactivities||Human (8), Dog (Canine) (7), Mouse (Murine) (7), Rat (Rattus) (7), All Species (2), Various Species (1)|
|Applications||Western Blotting (WB) (57), ELISA (52), Immunoprecipitation (IP) (35), Immunohistochemistry (IHC) (5), Immunofluorescence (IF) (2), Enzyme Immunoassay (EIA) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1)|
|Conjugates||HRP (9), Biotin (7)|
|Epitopes||pSer53 (3), pSer (2)|