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Thyroid Hormone Receptor Interactor 10 (TRIP10) (AA 411-501) antibody

Details for Product No. ABIN968877, Supplier: Log in to see
Antigen
  • CIP4
  • HSTP
  • STOT
  • STP
  • AI646975
  • Cip4
  • trip10
Epitope
AA 411-501
20
15
3
2
2
2
2
2
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
61
25
23
4
3
3
2
2
Host
Mouse
57
4
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
44
21
11
6
5
4
2
1
1
Supplier
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Immunogen Human CIP4 aa. 411-501
Clone 21-CIP4
Isotype IgG1
Cross-Reactivity Dog (Canine), Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Cip4 (TRIP10 Antibody Abstract)
Background Rho family members are small GTP binding proteins that serve as molecular switches for a number of biological processes. They cycle between active GTP-bound and inactive GDP-bound states. CDC42 is a Rho family protein that was identified in membranes of human platelets and placenta. It is the homologue of CDC42Sc, which regulates initiation of bud-site assembly in Saccharomyces cerevisiae. Similarly, CDC42 regulates the function of the mammalian actin cytoskeleton, allowing for efficient cytokinesis and cell morphogenesis. CDC42-interacting protein 4 (CIP4) was identified in a yeast-two hybrid screen for proteins that bind CDC42. Another variant of CIP4, CIP4/2, was identified that contains an extra 56 amino acids and has 71% identity with CIP4 (or CIP4/1). CIP4 contains a C-terminal SH3 domain and an N-terminal domain that is homologous to non-catalyitic motifs in the tyrosine kinase Fer. The mRNA expression of CIP4 is highest in skeletal muscle, heart, and placenta. Overexpression of CIP4 in Swiss 3T3 cells reduces the amount of stress fibers and leads to clustering of CIP4 to foci at the dorsal side of the cells. In addition, CIP4 binds the Rho-GTPase activating protein RICH and the cytoskeletal protein WASP. Coexpression of CIP4 and WASP in Cos-7 cells leads to WASP association with microtubules. Thus, CIP4 is involved in various protein-protein interactions associated with cytoskeletal dynamics. This antibody is routinely tested by western blot analysis.
Molecular Weight 80 kDa
Research Area Cancer, Cell Cycle, Cytoskeleton, Microfilaments
Pathways
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Thyroid Hormone Receptor Interactor 10 (TRIP10) (AA 411-501) antibody (ABIN968877) Western blot analysis of CIP4 on JAR lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1...
 image for anti-Thyroid Hormone Receptor Interactor 10 (TRIP10) (AA 411-501) antibody (ABIN968877) Immunofluroescent staining of NRK cells.
Product cited in: Richnau, Aspenström: "Rich, a rho GTPase-activating protein domain-containing protein involved in signaling by Cdc42 and Rac1." in: The Journal of biological chemistry, Vol. 276, Issue 37, pp. 35060-70, 2001 (PubMed).

Tian, Nelson, Stewart: "Cdc42-interacting protein 4 mediates binding of the Wiskott-Aldrich syndrome protein to microtubules." in: The Journal of biological chemistry, Vol. 275, Issue 11, pp. 7854-61, 2000 (PubMed).

Aspenström: "A Cdc42 target protein with homology to the non-kinase domain of FER has a potential role in regulating the actin cytoskeleton." in: Current biology : CB, Vol. 7, Issue 7, pp. 479-87, 1997 (PubMed).