RAB Guanine Nucleotide Exchange Factor (GEF) 1 (RABGEF1) (AA 426-481) antibody
Alternatives Western Blotting (WB)
|3 references available|
|Price||Product not available in this region.|
|Cross-Reactivity||Rat (Rattus), Human, Chicken|
The Rab proteins are small GTP-binding molecules. They are localized to specific intracellular vesicles and organelles and are important for vesicular trafficking, cycling between an active GTP-bound and inactive GDP-bound form. Rab5 is associated with vesicle trafficking between the early endosomes and plasma membrane. In vitro, Rab5 proteins are removed from membranes by a GDP dissociation inhibitor protein (rabGDI). This leads to the formation of a cytosolic Rab5-rabGDI complex. Rab5 may insert into membranes by a multistep process in which a transient GDP-Rab5 intermediate is formed and converted into GTP- Rab5. Rabaptin-5 interacts with GTP-Rab5, and is recruited to the endosomal fraction in a Rab5/GTP-dependent manner. Removal of Rabaptin-5 from the cytosol substantially impairs GTP and Rab5-dependent endosomal fusion. Rabex-5 forms a complex with Rabaptin-5 and displays GDP/GTP exchange activity on Rab5 that promotes interaction between Rabaptin-5 and Rab5. Rabex-5 is also known as Rin2 based on its 15% identitiy with the RasGTPase-binding protein Rin1. Thus, Rabex-5/Rabaptin-5 complex is critical for Rab5 GDP/GTP exchange and membrane-associated activity.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||60 kDa|
Related Products: ABIN968540, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20° C.|
|Restrictions||For Research Use only|
|Western blot analysis of Rabex-5 on a mouse cerebrum lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:2000 dilution of the mouse anti- Rabex-5 antibody.|
Horiuchi, Lippé, McBride et al.: "A novel Rab5 GDP/GTP exchange factor complexed to Rabaptin-5 links nucleotide exchange to effector recruitment and function." in: Cell, Vol. 90, Issue 6, pp. 1149-59, 1997 (PubMed).
Rubino, Miaczynska, Lippé et al.: "Selective membrane recruitment of EEA1 suggests a role in directional transport of clathrin-coated vesicles to early endosomes." in: The Journal of biological chemistry, Vol. 275, Issue 6, pp. 3745-8, 2000 (PubMed).
Saito, Murai, Kajiho et al.: "A novel binding protein composed of homophilic tetramer exhibits unique properties for the small GTPase Rab5." in: The Journal of biological chemistry, Vol. 277, Issue 5, pp. 3412-8, 2002 (PubMed).
|Reactivities||Human (12), Mouse (Murine) (5), Rat (Rattus) (5)|
|Applications||Western Blotting (WB) (13), ELISA (7), Immunohistochemistry (IHC) (5)|
|Epitopes||Internal Region (3), C-Term (1), Center (1), N-Term (1)|