alpha -Actinin (AA 629-825) antibody

Antigen: alpha -Actinin

Binding Site: AA 629-825

Clonality: Monoclonal (23)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN968886

Additional Information

Immunogen: Human alpha-Actinin

Cross-Reactivity: Chicken, Dog (Canine), Mouse (Murine), Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 23

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 104 kDa

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Restrictions: For Research Use only

Images

Western blot analysis of alpha-Actinin on jurkat lysate. Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of alpha-Actinin.

Publications

Product: Youssoufian, McAfee, Kwiatkowski: "Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene." in: American journal of human genetics, Vol. 47, Issue 1, pp. 62-72, 1990 (PubMed).

Viel: "Alpha-actinin and spectrin structures: an unfolding family story." in: FEBS letters, Vol. 460, Issue 3, pp. 391-4, 1999 (PubMed).

Izaguirre, Aguirre, Hu et al.: "The cytoskeletal/non-muscle isoform of alpha-actinin is phosphorylated on its actin-binding domain by the focal adhesion kinase." in: The Journal of biological chemistry, Vol. 276, Issue 31, pp. 28676-85, 2001 (PubMed).