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DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 476-670) antibody

Details for Product No. ABIN968896, Supplier: Login to see
Antigen
  • cb1075
  • cb983
  • dmnt1
  • fb05h01
  • mta
  • wu:fb05h01
  • ADCADN
  • AIM
  • CXXC9
  • DNMT
  • HSN1E
  • MCMT
  • DDM2
  • DECREASED DNA METHYLATION 2
  • DMT01
  • DMT1
  • DNA METHYLTRANSFERASE
  • DNA METHYLTRANSFERASE 01
  • DNA METHYLTRANSFERASE 1
  • MET2
  • METHYLTRANSFERASE 2
  • METHYLTRANSFERASE I
  • METI
  • methyltransferase 1
  • Cxxc9
  • Dnmt
  • Dnmt1o
  • MTase
  • Met-1
  • Met1
  • MommeD2
  • m.MmuI
Epitope
AA 476-670
59
42
35
28
22
22
20
19
13
13
13
12
10
9
9
7
7
7
6
5
5
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
387
110
41
40
36
5
5
5
3
2
1
1
1
1
1
1
Host
Mouse
252
110
20
8
3
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
23
23
20
18
18
18
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
Application
Immunofluorescence (IF), Western Blotting (WB)
255
130
104
97
82
72
46
39
25
22
10
7
2
1
1
Supplier
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Immunogen Human DNMT1 aa. 476-670
Clone 18-DNMT1
Isotype IgG2b
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name DNMT1 (DNMT1 Antibody Abstract)
Background DNA methylation of the 5' position of the cytosine ring within CpG dinucleotides is essential for embryonic development. This methylation may affect transcriptional repression, formation of compact chromatin structures, X chromosome inactivation, and imprinting control. Three important DNA (Cytosine-5) methyltransferases (DNMTs) include DNMT1, DNMT3A, and DNMT3B. DNMT1 contains an N-terminal regulatory domain that may target it to replication foci, and a C-terminal catalytic domain that is homologous to bacterial cystosine 5-methylases. DNMT1 knockout mice have reduced DNA methylation, defects in imprinting, and derepression of endogenous retroviruses, and are embryonic lethal. DNMT1 is responsible for DNA methylations that occur after replication, while DNMT3A and DNMT3B act to establish new methylation patterns during embryogenesis. DNMT1 and DNMT3A are expressed in most fetal tissues, while DNMT3B is expressed only in fetal liver. All of these DNMTs are found in various adult tissues and show increased expression in some tumors and cancer cell lines. Thus, DNMT methylation of CpG dinucleotides is important for regulating DNA structure during development. This antibody is routinely tested by western blot analysis.
Synonyms: DNA Methyl Transferase-1
Molecular Weight 200 kDa
Research Area DNA/RNA
Pathways
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 476-670) antibody (ABIN968896) Western blot analysis of DNMT1 on a Jurkat cell lysate (Human T-cell leukemia, ATCC T...
Immunofluorescence (IF) image for anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 476-670) antibody (ABIN968896) Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma, ATCC ...
Product cited in: Robertson, Ait-Si-Ali, Yokochi et al.: "DNMT1 forms a complex with Rb, E2F1 and HDAC1 and represses transcription from E2F-responsive promoters." in: Nature genetics, Vol. 25, Issue 3, pp. 338-42, 2000 (PubMed).

Robertson, Uzvolgyi, Liang et al.: "The human DNA methyltransferases (DNMTs) 1, 3a and 3b: coordinate mRNA expression in normal tissues and overexpression in tumors." in: Nucleic acids research, Vol. 27, Issue 11, pp. 2291-8, 1999 (PubMed).

Yoder, Yen, Vertino et al.: "New 5' regions of the murine and human genes for DNA (cytosine-5)-methyltransferase." in: The Journal of biological chemistry, Vol. 271, Issue 49, pp. 31092-7, 1997 (PubMed).

Yen, Vertino, Nelkin et al.: "Isolation and characterization of the cDNA encoding human DNA methyltransferase." in: Nucleic acids research, Vol. 20, Issue 9, pp. 2287-91, 1992 (PubMed).