DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 476-670) antibody

Details for Product No. ABIN968896
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Antigen
Synonyms
cb1075, cb983, dmnt1, fb05h01, mta, wu:fb05h01, ADCADN, AIM, CXXC9, DNMT, HSN1E, MCMT, DDM2, DECREASED DNA METHYLATION 2, DMT01, DMT1, DNA METHYLTRANSFERASE, DNA METHYLTRANSFERASE 01, DNA METHYLTRANSF ... show more
cb1075, cb983, dmnt1, fb05h01, mta, wu:fb05h01, ADCADN, AIM, CXXC9, DNMT, HSN1E, MCMT, DDM2, DECREASED DNA METHYLATION 2, DMT01, DMT1, DNA METHYLTRANSFERASE, DNA METHYLTRANSFERASE 01, DNA METHYLTRANSFERASE 1, MET2, METHYLTRANSFERASE 2, METHYLTRANSFERASE I, METI, methyltransferase 1, Cxxc9, Dnmt, Dnmt1o, MTase, Met-1, Met1, MommeD2, m.MmuI show less
Epitope
AA 476-670
(23), (20), (17), (16), (13), (13), (12), (12), (12), (11), (11), (8), (7), (6), (6), (5), (5), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(227), (64), (57), (52), (42), (33), (24), (6), (2), (1), (1), (1), (1)
Host
Mouse
(167), (51), (10), (7), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(13), (13), (11), (8), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunofluorescence (IF)
(148), (70), (60), (50), (44), (42), (38), (38), (12), (12), (9), (1), (1)
Pubmed 4 references available
Quantity 150 μg
Options
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Catalog No. ABIN968896
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Immunogen Human DNMT1
Clone 18
Isotype IgG2b
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name DNMT1
Background DNA methylation of the 5' position of the cytosine ring within CpG dinucleotides is essential for embryonic development. This methylation may affect transcriptional repression, formation of compact chromatin structures, X chromosome inactivation, and imprinting control. Three important DNA (Cytosine-5) methyltransferases (DNMTs) include DNMT1, DNMT3A, and DNMT3B. DNMT1 contains an N-terminal regulatory domain that may target it to replication foci, and a C-terminal catalytic domain that is homologous to bacterial cystosine 5-methylases. DNMT1 knockout mice have reduced DNA methylation, defects in imprinting, and derepression of endogenous retroviruses, and are embryonic lethal. DNMT1 is responsible for DNA methylations that occur after replication, while DNMT3A and DNMT3B act to establish new methylation patterns during embryogenesis. DNMT1 and DNMT3A are expressed in most fetal tissues, while DNMT3B is expressed only in fetal liver. All of these DNMTs are found in various adult tissues and show increased expression in some tumors and cancer cell lines. Thus, DNMT methylation of CpG dinucleotides is important for regulating DNA structure during development. This antibody is routinely tested by western blot analysis.
Synonyms: DNA Methyl Transferase-1
Molecular Weight 200 kDa
Research Area DNA/RNA
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 476-670) antibody Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma, ATCC CCL-2.2).
anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (AA 476-670) antibody (2) Western blot analysis of DNMT1 on a Jurkat cell lysate (Human T-cell leukemia, ATCC TIB-152). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti- human DNMT1 antibody.
Product cited in: Yen, Vertino, Nelkin et al.: "Isolation and characterization of the cDNA encoding human DNA methyltransferase." in: Nucleic acids research, Vol. 20, Issue 9, pp. 2287-91, 1992 (PubMed).

Yoder, Yen, Vertino et al.: "New 5' regions of the murine and human genes for DNA (cytosine-5)-methyltransferase." in: The Journal of biological chemistry, Vol. 271, Issue 49, pp. 31092-7, 1997 (PubMed).

Robertson, Uzvolgyi, Liang et al.: "The human DNA methyltransferases (DNMTs) 1, 3a and 3b: coordinate mRNA expression in normal tissues and overexpression in tumors." in: Nucleic acids research, Vol. 27, Issue 11, pp. 2291-8, 1999 (PubMed).

Robertson, Ait-Si-Ali, Yokochi et al.: "DNMT1 forms a complex with Rb, E2F1 and HDAC1 and represses transcription from E2F-responsive promoters." in: Nature genetics, Vol. 25, Issue 3, pp. 338-42, 2000 (PubMed).

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