Actin antibody

Details for Product No. ABIN968903
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Antigen
Synonyms
Act57, Act57A, Act57b, Actin, BEST:RE38067, C, CG10067, CG30294, Dmel\\CG10067, M, T10, act 57A, act57A, act57B, act57b, actin, anon-EST:CL2c12, 143060_f_at, CG7478, D, Dmel\\CG7478, act 79B, act79B,  ... show more
Act57, Act57A, Act57b, Actin, BEST:RE38067, C, CG10067, CG30294, Dmel\\CG10067, M, T10, act 57A, act57A, act57B, act57b, actin, anon-EST:CL2c12, 143060_f_at, CG7478, D, Dmel\\CG7478, act 79B, act79B, mus79B, Act(88F), Act88-F, Act88f, CG5178, Dmel\\CG5178, E, Ifm(3)7, act 88F, act88F, rsd, Act87e, CG18290, Dmel\\CG18290, act 87E, act87E, mus87E, Act88F, Dsim\\GD19025, GD19025, dsim_GLEANR_2829, 42A, A, AFFX-Dros-ACTIN_M_r_at, Act, Act42, Act42a, Actin/BAP47, BAP47, CG12051, Dmel\\CG12051, act 42A, act42A show less
Reactivity
Chicken
(106), (63), (63), (42), (19), (15), (14), (12), (8), (7), (7), (7), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Mouse
(93), (55), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(9), (7), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(105), (49), (47), (37), (29), (25), (20), (16), (13), (2), (2), (1), (1)
Pubmed 4 references available
Catalog no. ABIN968903
Quantity 150 µg
Price
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Immunogen Chicken gizzard muscle Actin
Clone C4
Isotype IgG1
Cross-Reactivity Human, Dog (Canine), Rat (Rattus), Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Background Changes in cellular morphology, adhesion, and motility occur through the reorganization of the actin cytoskeleton. This reorganization of actin filaments results from interactions between actin and actin-binding proteins. Actin is a 42-kDa protein that is known as G-actin in its monomeric form. Polymerization of G-actin monomers leads to the generation of flexible filaments, 5-9 nm in diameter, called F-actin. F-actin may be organized in linear bundles called stress fibers or in two-dimensional networks. The latter are highly concentrated beneath the plasma membrane and form the actin cortex. Regulation of actin cytoskeletal dynamics occurs through actin-binding proteins. These proteins bind to G- and/or F-actin and regulate various aspects of actin cytoskeletal dynamics, such as polymerization and depolymerization of actin, cross-linking of actin filaments into bundles, interaction of actin-based structures with membranes and other cytoskeletal elements, and locomotion of actin-based structures. Thus, the actin cytoskeleton is a complex matrix consisting of G- and F-actin along with the multitude of interactions between these actin forms and a variety of different types of actin-binding proteins. The C4 monoclonal antibody reacts with all known isoforms of actin in vertebrate muscle and non-muscle cells.
Molecular Weight 42 kDa
Research Area Cancer, Apoptosis/Necrosis, Cytoskeleton, Microfilaments, Extracellular Matrix
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Storage -20 °C
Supplier Images
anti-Actin antibody Western blot analysis of Actin Ab-5 on Jurkat cell lysate. Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of anti-Actin Ab-5.
anti-Actin antibody (2) Immunofluorescent staining of Hs68 cells with anti-Actin Ab-5.
Product cited in: Hanstein, Lange, Schneider-Poetsch et al.: "Detection of actin and localization of phytochrome in the green alga Mougeotia by monoclonal antibodies." in: Acta histochemica. Supplementband, Vol. 41, Issue 9, pp. 223-30, 1992 (PubMed).

Lessard: "Two monoclonal antibodies to actin: one muscle selective and one generally reactive." in: Cell motility and the cytoskeleton, Vol. 10, Issue 3, pp. 349-62, 1988 (PubMed).

Mitchison, Cramer: "Actin-based cell motility and cell locomotion." in: Cell, Vol. 84, Issue 3, pp. 371-9, 1996 (PubMed).

Pantaloni, Le Clainche, Carlier: "Mechanism of actin-based motility." in: Science (New York, N.Y.), Vol. 292, Issue 5521, pp. 1502-6, 2001 (PubMed).

Hosts (93), (55), (2), (1)
Reactivities (106), (63), (63), (42), (19), (15), (14), (12), (8), (7), (7), (7), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applications (105), (49), (47), (37), (29), (25), (20), (16), (13), (2), (2), (1), (1)
Conjugates (9), (7), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Epitopes (17), (8), (5), (5), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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