Fyn/c-Src (pTyr528,pTyr530) antibody

Antigen: Fyn/c-Src

Binding Site: pTyr528,pTyr530

Clonality: Monoclonal (31)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN968908

Additional Information

Immunogen: Phosphorylated Human Fyn (Y528) Peptide

Cross-Reactivity: Mouse (Murine)

Format: Liquid

Isotype: IgG2b

Clone: 31

Description: P59fyn is a member of the Src family of protein tyrosine kinases. Two isoforms of the protein have been identified, designated as FynB and Fyn T. Fyn B has been shown to be localized to the brain, whereas Fyn T associates with both B and T cells. Stimulation of the T cell antigen receptor (TcR) results in protein tyrosine phosphorylation via non-receptor tyrosine kinases. p59fyn kinase (Fyn T) associates with the TcR. Ligation of the TcR activates the protein kinase activity of p59fyn in various human T cells. Fyn interacts with the CD3-zeta chains through its N-terminal region. In turn, fyn binds other proteins through its SH2 and SH3 domains. These proteins (p82 and p116) may serve as substrates and/or mediators of fyn activity. p59fyn kinase is tyrosine phosphorylated at two sites, Tyr-417 (autophosphorylation site), and Tyr-528 (negative regulatory site).

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 60 kDa

Comments:

Related Products: ABIN968534, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Restrictions: For Research Use only

Images

Western blot analysis for Fyn (pY528) / c-Src (pY530). A431 cells (Human epithelial carcinoma, ATCC CRL-1555) were treated with 100 ng/ml EGF for 5 min and then either left untreated (lane 1) or treated (lane 2) with 50 µg/ml alkaline phosphatase for 30 min at 37°C. The top panel was probed with a mouse anti-Src antibody and the bottom was probed with the mouse anti-Fyn (pY528) / c-Src (pY530) antibody at a 1:2500 dilution with a band observable at ~ 60 kDa.

Publications

Product: Grant, ODell, Karl et al.: "Impaired long-term potentiation, spatial learning, and hippocampal development in fyn mutant mice." in: Science (New York, N.Y.), Vol. 258, Issue 5090, pp. 1903-10, 1993 (PubMed).

Cooke, Perlmutter: "Expression of a novel form of the fyn proto-oncogene in hematopoietic cells." in: The New biologist, Vol. 1, Issue 1, pp. 66-74, 1991 (PubMed).

Kawakami, Kawakami, Aaronson et al.: "Acquisition of transforming properties by FYN, a normal SRC-related human gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 11, pp. 3870-4, 1988 (PubMed).