Fyn/c-Src (pTyr528,pTyr530) antibody
Western Blotting (WB)
|3 references available|
|Quantity||50 µg (250 µg/ml)|
|Price||Product not available in this region.|
|Immunogen||Phosphorylated Human Fyn (Y528) Peptide|
|Description||P59fyn is a member of the Src family of protein tyrosine kinases. Two isoforms of the protein have been identified, designated as FynB and Fyn T. Fyn B has been shown to be localized to the brain, whereas Fyn T associates with both B and T cells. Stimulation of the T cell antigen receptor (TcR) results in protein tyrosine phosphorylation via non-receptor tyrosine kinases. p59fyn kinase (Fyn T) associates with the TcR. Ligation of the TcR activates the protein kinase activity of p59fyn in various human T cells. Fyn interacts with the CD3-zeta chains through its N-terminal region. In turn, fyn binds other proteins through its SH2 and SH3 domains. These proteins (p82 and p116) may serve as substrates and/or mediators of fyn activity. p59fyn kinase is tyrosine phosphorylated at two sites, Tyr-417 (autophosphorylation site), and Tyr-528 (negative regulatory site).|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||60 kDa|
Related Products: ABIN968534, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20°C.|
|Restrictions||For Research Use only|
Grant, ODell, Karl et al.: "Impaired long-term potentiation, spatial learning, and hippocampal development in fyn mutant mice." in: Science (New York, N.Y.), Vol. 258, Issue 5090, pp. 1903-10, 1993 (PubMed).
Cooke, Perlmutter: "Expression of a novel form of the fyn proto-oncogene in hematopoietic cells." in: The New biologist, Vol. 1, Issue 1, pp. 66-74, 1991 (PubMed).
Kawakami, Kawakami, Aaronson et al.: "Acquisition of transforming properties by FYN, a normal SRC-related human gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 11, pp. 3870-4, 1988 (PubMed).