LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1) (AA 120-220) antibody

Antigen: LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1)

Binding Site: AA 120-220

Clonality: Monoclonal (49)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN968920

Additional Information

Alternative name: PINCH

Immunogen: Human PINCH

Cross-Reactivity: Rat (Rattus), Mouse (Murine)

No Cross-Reactivity: Dog (Canine), Chicken

Format: Liquid

Isotype: IgG2a

Clone: 49

Description: Integrins are transmembrane receptors that mediate cell-cell or cell-matrix adhesion. All integrins are heterodimers composed of alpha and beta subunits, which interact with extracellular matrix and cytoskeletal proteins. Signal transduction through intergin receptors may be regulated by integrin-linked kinase (ILK). ILK is a widely expressed Ser/Thr protein kinase that contains four ankyrin-like repeats in the N-terminal region, a phoshoinositide lipid-binding motif at amino acids 180-212, and an integrin binding site at amino acids 293-451. The ankyrin repeats interact with a LIM domain-only protein called PINCH that also binds the adaptor protein, Nck-2. This interaction implicates ILK in growth factor receptor pathways. The PINCH adaptor protein also contains a leucine-rich nuclear export signal and a nuclear localization signal. These two motifs implicate PINCH in nuclear functions, and in Schwann cells PINCH has been found in the nucleus, as well as, cytoplasm and perinuclear regions. Thus, PINCH is an adaptor protein that functions in both integrin signaling and nuclear functions.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 37 kDa

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of PINCH on K-562 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-PINCH antibody.

Publications

Product: Tu, Li, Wu: "Nck-2, a novel Src homology2/3-containing adaptor protein that interacts with the LIM-only protein PINCH and components of growth factor receptor kinase-signaling pathways." in: Molecular biology of the cell, Vol. 9, Issue 12, pp. 3367-82, 1999 (PubMed).

Tu, Li, Goicoechea et al.: "The LIM-only protein PINCH directly interacts with integrin-linked kinase and is recruited to integrin-rich sites in spreading cells." in: Molecular and cellular biology, Vol. 19, Issue 3, pp. 2425-34, 1999 (PubMed).

Campana, Myers, Rearden: "Identification of PINCH in Schwann cells and DRG neurons: shuttling and signaling after nerve injury." in: Glia, Vol. 41, Issue 3, pp. 213-23, 2003 (PubMed).