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PKC theta antibody (pThr538)

PRKCQ Reactivity: Human WB, IHC (f) Host: Mouse Monoclonal 19-PKC unconjugated
Catalog No. ABIN968924
  • Target See all PKC theta (PRKCQ) Antibodies
    PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))
    Binding Specificity
    • 30
    • 28
    • 15
    • 13
    • 12
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pThr538
    Reactivity
    • 94
    • 66
    • 54
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    Human
    Host
    • 121
    • 2
    • 2
    • 1
    Mouse
    Clonality
    • 123
    • 3
    Monoclonal
    Conjugate
    • 60
    • 7
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    This PKC theta antibody is un-conjugated
    Application
    • 107
    • 47
    • 40
    • 40
    • 38
    • 24
    • 11
    • 10
    • 5
    • 5
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Phosphorylated Human PKCtheta Peptide
    Clone
    19-PKC
    Isotype
    IgG2a
    Top Product
    Discover our top product PRKCQ Primary Antibody
  • Comment

    Related Products: ABIN967738

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20°C.
  • Soderling: "Protein kinases. Regulation by autoinhibitory domains." in: The Journal of biological chemistry, Vol. 265, Issue 4, pp. 1823-6, (1990) (PubMed).

    Nishizuka: "The molecular heterogeneity of protein kinase C and its implications for cellular regulation." in: Nature, Vol. 334, Issue 6184, pp. 661-5, (1988) (PubMed).

  • Target
    PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))
    Alternative Name
    PKC theta (PRKCQ Products)
    Synonyms
    PRKCT antibody, nPKC-theta antibody, A130035A12Rik antibody, AW494342 antibody, PKC-0 antibody, PKC-theta antibody, PKCtheta antibody, Pkcq antibody, protein kinase C theta antibody, protein kinase C eta antibody, protein kinase C, theta antibody, PRKCQ antibody, PRKCH antibody, Prkcq antibody
    Background
    The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. Three categories exist, conventional PKC (cPKC), novel PKC (nPKC), and atypical PKC (aPKC). These proteins are products of multiple genes and alternative splicing and have different modes of activation. For example, cPKC's members (alpha, betaI, betaII, and gamma) are calcium activated, phospholipid-dependent serine/threonine specific enzymes which can also be activated by phorbol esters. However, the novel PKC (nPKC) subfamily members (delta , epsilon, pi, and theta isoforms) and the atypical PKC (PKC) subfamily members (zeta , í , and lambda isoforms) are Ca[2+] independent. The aPKC members are unique in that their activity is independent of diacylglycerols and phorbol esters. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. PKCtheta transcripts are expressed in most tissues with the highest levels being found in hematopoietic tissues and cell lines, including T cells and thymocytes. PKCtheta RNA is readily detectable in skeletal muscle, lung, and brain. However, PKCtheta expression is not detected in several human carcinoma cell lines. Abundant expression of this PKC isozyme in hematopoietic cells suggests that it may have a role in growth and differentiation processes of these cells.
    The 9/PKC monoclonal antibody recognizes the phosphorylated threonine 538 (pT538) of human PKCtheta.
    Molecular Weight
    79 kDa
    Pathways
    TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Thromboxane A2 Receptor Signaling
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