STAT4 antibody (pTyr693)

Catalog No. ABIN968926

Product Details anti-STAT4 Antibody

Target: STAT4 (Signal Transducer and Activator of Transcription 4 (STAT4))

Binding Specificity: pTyr693

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This STAT4 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human Stat4 (pY693)

Clone: 38-p

Isotype: IgG2b kappa

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-STAT4 antibody (ABIN968926)

Kisseleva, Bhattacharya, Braunstein, Schindler: "Signaling through the JAK/STAT pathway, recent advances and future challenges." in: Gene, Vol. 285, Issue 1-2, pp. 1-24, (2002) (PubMed).

Visconti, Gadina, Chiariello, Chen, Stancato, Gutkind, OShea: "Importance of the MKK6/p38 pathway for interleukin-12-induced STAT4 serine phosphorylation and transcriptional activity." in: Blood, Vol. 96, Issue 5, pp. 1844-52, (2000) (PubMed).

Target Details for STAT4

Target: STAT4 (Signal Transducer and Activator of Transcription 4 (STAT4))

Alternative Name: Stat4 (STAT4 Products)

Synonyms: SLEB11 antibody, signal transducer and activator of transcription 4 antibody, STAT4 antibody, stat4 antibody, Stat4 antibody

Background: The Stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Seven mammalian Stat proteins have been identified: Stat1-4, Stat5a, 5b, and Stat6. Stat4 has been shown to play an important role in development of T helper cells, specifically the Th1 subset. Stat4 is activated by IL-12 and by type 1 interferons. Knockout mice supported the role that Stat4 plays in IL-12 signaling because lymphocytes from Stat 4-/- mice could not differentiate into Th1 cells or produce IFNgamma in response to treatment with IL-12. IFNgamma plays an important role in host defense. A key component in the activation of Stat4 is the phosphorylation on tyrosine and serine residues in response to IL-12 stimulation. IL-12 stimulation leads to the phosphorylation of Stat4 on tyrosine 693 and serine 721. Transcriptional activity of Stat4 has been shown to be significantly reduced when residues Y693 and S721 are mutated.

Molecular Weight: 89 kDa

Pathways: JAK-STAT Signaling