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EIF4EBP1 antibody (Eukaryotic Translation Initiation Factor 4E Binding Protein 1)

Details for Product anti-EIF4EBP1 Antibody No. ABIN968937, Supplier: Log in to see
Antigen
  • 4e-bp
  • 4E-BP
  • 4e-bp1
  • 4E-BP1
  • 4ebp
  • 4eBP
  • 4EBP
  • 4EBP1
  • 43-BP
  • 153432_at
  • AA959816
  • anon-WO0172774.158
  • BcDNA.HL08053
  • BcDNA:HL08053
  • BP-1
  • CG8846
  • CT8705
  • d4e-bp
  • d4E-BP
  • d4E-BP1
  • d4EBP
  • d4eBP
  • Dm 4E-BP
  • Dmel\\CG8846
  • dthor
  • eIF4E-BP
  • EIF4EBP1
  • fc04h09
  • l(2)06270
  • l(2)k07736
  • l(2)k13517
  • PHAS-I
  • Phas1
  • pp20
  • thor
  • Thor/EIF4EBP
  • wu:fc04h09
  • zgc:64137
Alternatives
anti-Human EIF4EBP1 antibody for Western Blotting
Reactivity
Human
438
240
192
11
8
5
2
2
1
1
1
Host
Mouse
386
91
9
Clonality (Clone)
Monoclonal ()
Conjugate
This EIF4EBP1 antibody is un-conjugated
20
17
12
11
8
8
5
5
5
5
5
5
5
5
5
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
316
202
192
89
56
27
21
18
15
7
5
4
4
3
3
1
Supplier
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Supplier Product No.
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Immunogen Purified recombinant fragment of 4EBP1 expressed in E. coli.
Clone 9E12D9
Isotype IgG1
Purification Ascites
Alternative Name 4E-BP1 (EIF4EBP1 Antibody Abstract)
Background 4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1),also called ELF4EBP1/BP-1/PHAS-I ,which is located on chromosome 8p12, with 118-amino acid protein (about 13 kDa). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while(24 kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.
Gene ID 1978
HGNC 1978
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
Western Blotting (WB) image for anti-EIF4EBP1 antibody (Eukaryotic Translation Initiation Factor 4E Binding Protein 1) (ABIN968937) Figure 1: Western blot analysis using 4E-BP1 mouse mAb against truncated 4E-BP1 recom...
Background publications Fadden, Haystead, Lawrence: "Identification of phosphorylation sites in the translational regulator, PHAS-I, that are controlled by insulin and rapamycin in rat adipocytes." in: The Journal of biological chemistry, Vol. 272, Issue 15, pp. 10240-7, 1997 (PubMed).

Pause, Belsham, Gingras, Donzé, Lin, Lawrence, Sonenberg: "Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5'-cap function." in: Nature, Vol. 371, Issue 6500, pp. 762-7, 1994 (PubMed).