Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) antibody

Details for Product No. ABIN968945
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Antigen
Synonyms ASMA, SMactin, a-sma, aat6, actsa, mymy5, sm-actin, ACTA2, actc1, ACT-4, AAT6, ACTSA, MYMY5, 0610041G09Rik, Actvs, SMalphaA, a-SMA, alphaSMA, wu:fb63d03, asma
Reactivity
Human, Monkey, Rat (Rattus), Mouse (Murine)
(146), (53), (41), (14), (14), (10), (7), (7), (6), (3), (3), (2), (2), (1), (1), (1)
Host
Mouse
(84), (66), (6)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(6), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Fluorescence Microscopy (FM)
(101), (67), (60), (48), (32), (21), (20), (19), (6), (1), (1), (1), (1), (1)
Pubmed 2 references available
Quantity 100 μL
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Catalog No. ABIN968945
396.00 $
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  • +1 404 474 4654
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Immunogen Purified recombinant fragment of human ACTA2 expressed in E. coli.
Clone 1H8
Isotype IgG1
Purification Ascites
Alternative Name ACTA2
Background Synonyms:
AAT6, ACTSA, ACTA2
Actin,α 2, smooth muscle, aorta, major constituent of thin filaments.
Molecular Weight 42 kDa
Gene ID 59
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) antibody Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 ng), Blue: Antigen (100 ng)
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) antibody (2) Figure 3: Flow cytometric analysis of Hela cells using ACTA2 mouse mAb (green) and negative control (purple) .
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) antibody (3) Figure 2: Immunofluorescence analysis of NIH/3T3 cells using ACTA2 mouse mAb (green) . Blue: DRAQ5 fluorescent DNA dye.
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) antibody (4) Figure 1: Western blot analysis using ACTA2 mouse mAb against Hela (1), Jurkta (2), HepG2 (3), MCF-7 (4), A431 (5), A549 (6), PC-12 (7), NIH/3T3 (8) and Cos7 (9) cell lysate.
Product cited in: Salas, Bartoli, Deville et al.: "Ezrin and alpha-smooth muscle actin are immunohistochemical prognostic markers in conventional osteosarcomas." in: Virchows Archiv : an international journal of pathology, Vol. 451, Issue 6, pp. 999-1007, 2007 (PubMed).

Guo, Pannu, Tran-Fadulu et al.: "Mutations in smooth muscle alpha-actin (ACTA2) lead to thoracic aortic aneurysms and dissections." in: Nature genetics, Vol. 39, Issue 12, pp. 1488-93, 2007 (PubMed).

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