anti-beta Actin antibody (Actin, beta) (N-Term)

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Antigen
Synonyms
beta-actin, ps1tp5bp1, LOC563571, ACTB, A26C1A, A26C1B, act-2, actb, B-actin, LOC100136013, LOC100174883, LOC100304816, A, ACT5C, Ac5C, Act, Act-5C, Act5, Act5c, Actin, Actin/BAP47, Actin5C, BAP47, Ba ... show more
beta-actin, ps1tp5bp1, LOC563571, ACTB, A26C1A, A26C1B, act-2, actb, B-actin, LOC100136013, LOC100174883, LOC100304816, A, ACT5C, Ac5C, Act, Act-5C, Act5, Act5c, Actin, Actin/BAP47, Actin5C, BAP47, Bap47, CG4027, Dmel\\CG4027, M32055, T11, act, act 5C, act5C, actin, actin5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, Bact, BRWS1, PS1TP5BP1, ACT-5, Actx, E430023M04Rik, ACTA1, Beta-actin, B-ACTZF, actba, bact, bactin1, bactzf, wu:fd18f05, POTE2alpha, POTEACTIN show less
Epitope
N-Term
(133), (38), (15), (14), (11), (7), (6), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Hamster, Rat (Rattus), Monkey
(492), (199), (173), (57), (57), (44), (37), (35), (31), (26), (24), (16), (12), (12), (10), (9), (7), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Mouse
(316), (203), (19), (1), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
This beta Actin antibody is un-conjugated
(29), (19), (15), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
(464), (209), (145), (90), (87), (46), (40), (20), (12), (12), (11), (11), (9), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Quantity 100 μL
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Immunogen Synthetic peptide corresponding to amino-terminal residues of human beta-Actin conjugated to KLH.
Clone 8H10D10
Isotype IgG2b
Purification Ascites
Alternative Name beta-Actin (ACTB Antibody Abstract)
Background Beta-actin (PS1TP5-binding protein 1), also known as ACTB, PS1TP5BP1. Entrez Protein NP_001092. It is one of six different actin proteins. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton.Actins are highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Actin is ubiquitously expressed in all eukaryotic cells. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Molecular Weight 42 kDa
Gene ID 60
HGNC 60
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
Flow Cytometry (FACS) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN968977) Figure 1: Flow cytometric analysis of MCF-7 cells using beta Actin mouse mAb (right) ...
Immunocytochemistry (ICC) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN968977) Figure 2: Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cell...
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN968977) Figure 3: Western blot analysis using beta-Actin mouse mAb against NIH/3T3 (1), Jurka...
Product cited in: Rual, Venkatesan, Hao et al.: "Towards a proteome-scale map of the human protein-protein interaction network. ..." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).

Pederson, Aebi: "Nuclear actin extends, with no contraction in sight." in: Molecular biology of the cell, Vol. 16, Issue 11, pp. 5055-60, 2005 (PubMed).

Xu, Wratten, Charych et al.: "Increased expression in dorsolateral prefrontal cortex of CAPON in schizophrenia and bipolar disorder." in: PLoS medicine, Vol. 2, Issue 10, pp. e263, 2005 (PubMed).

Bruneel, Labas, Mailloux et al.: "Proteomics of human umbilical vein endothelial cells applied to etoposide-induced apoptosis." in: Proteomics, Vol. 5, Issue 15, pp. 3876-84, 2005 (PubMed).

Catalog No. ABIN968977
$393.80
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