Actin, beta (ACTB) (N-Term) antibody

Details for Product No. ABIN968977
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Antigen
Synonyms
beta-actin, ps1tp5bp1, LOC563571, ACTB, A26C1A, A26C1B, act-2, actb, B-actin, LOC100136013, LOC100174883, LOC100304816, A, ACT5C, Ac5C, Act, Act-5C, Act5, Act5c, Actin, Actin/BAP47, Actin5C, BAP47, Ba ... show more
beta-actin, ps1tp5bp1, LOC563571, ACTB, A26C1A, A26C1B, act-2, actb, B-actin, LOC100136013, LOC100174883, LOC100304816, A, ACT5C, Ac5C, Act, Act-5C, Act5, Act5c, Actin, Actin/BAP47, Actin5C, BAP47, Bap47, CG4027, Dmel\\CG4027, M32055, T11, act, act 5C, act5C, actin, actin5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, Bact, BRWS1, PS1TP5BP1, ACT-5, Actx, E430023M04Rik, ACTA1, Beta-actin, B-ACTZF, actba, bact, bactin1, bactzf, wu:fd18f05, POTE2alpha, POTEACTIN show less
Epitope
N-Term
(110), (34), (12), (9), (6), (4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Hamster, Rat (Rattus), Monkey
(351), (188), (175), (67), (49), (44), (40), (32), (30), (25), (22), (21), (12), (12), (11), (7), (7), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host
Mouse
(218), (141), (20)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(17), (12), (6), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application
ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Fluorescence Microscopy (FM)
(337), (149), (92), (77), (64), (57), (27), (27), (20), (10), (9), (4), (3), (2), (2), (1)
Pubmed 3 references available
Quantity 100 μL
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Catalog No. ABIN968977
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Immunogen Synthetic peptide corresponding to amino-terminal residues of human beta-Actin conjugated to KLH.
Clone 8H10D10
Isotype IgG2b
Purification Ascites
Alternative Name beta-Actin
Background Synonyms:
PS1TP5BP1, ACTB
β-actin (PS1TP5-binding protein 1), also known as ACTB, PS1TP5BP1. Entrez Protein NP_001092. It is one of six different actin proteins. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. Actins are highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Actin is ubiquitously expressed in all eukaryotic cells. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskelηl actins.
Molecular Weight 42 kDa
Gene ID 60
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-Actin, beta (ACTB) (N-Term) antibody Figure 3: Flow cytometric analysis of MCF-7 cells using beta Actin mouse mAb (right) and negative control (left) .
anti-Actin, beta (ACTB) (N-Term) antibody (2) Figure 2: Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using beta Actin mouse mAb (red, the secondary Ab is Cy3-Goat anti mouse IgG) . Blue: DRAQ5 fluorescent DNA dye.
anti-Actin, beta (ACTB) (N-Term) antibody (3) Figure 1: Western blot analysis using beta-Actin mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), CHO (4), PC12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysate.
Product cited in: Xu, Wratten, Charych et al.: "Increased expression in dorsolateral prefrontal cortex of CAPON in schizophrenia and bipolar disorder." in: PLoS medicine, Vol. 2, Issue 10, pp. e263, 2005 (PubMed).

Pederson, Aebi: "Nuclear actin extends, with no contraction in sight." in: Molecular biology of the cell, Vol. 16, Issue 11, pp. 5055-60, 2005 (PubMed).

Rual, Venkatesan, Hao et al.: "Towards a proteome-scale map of the human protein-protein interaction network." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).

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