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Bruton Agammaglobulinemia tyrosine Kinase (BTK) antibody

Details for Product No. ABIN968992, Supplier: Log in to see
Antigen
  • BTK
  • atk
  • bpk
  • xla
  • imd1
  • agmx1
  • psctk1
  • AGMX1
  • AT
  • ATK
  • BPK
  • IMD1
  • PSCTK1
  • XLA
  • AI528679
  • xid
Alternatives
anti-Human Bruton Agammaglobulinemia tyrosine Kinase antibody for Immunoprecipitation
Reactivity
Human, Monkey
240
93
79
13
6
4
2
1
Host
Mouse
172
68
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
10
8
7
5
4
4
4
4
3
3
3
3
3
3
3
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
181
90
76
57
42
35
25
14
10
5
2
2
2
2
Supplier
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Immunogen Purified recombinant fragment of BTK expressed in E. coli.
Clone 7F12H4
Isotype IgG1
Purification Ascites
Alternative Name BTK (BTK Antibody Abstract)
Background Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases.All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini.BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation.
Molecular Weight 77 kDa
Gene ID 695
HGNC 695
Research Area Immunology, Innate Immunity, Tyrosine Kinases
Pathways Fc-epsilon Receptor Signaling Pathway
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
Immunocytochemistry (ICC) image for anti-Bruton Agammaglobulinemia tyrosine Kinase (BTK) antibody (ABIN968992) Figure 1: Immunofluorescence analysis of Jurkat cells using BTK mouse mAb.
Immunohistochemistry (IHC) image for anti-Bruton Agammaglobulinemia tyrosine Kinase (BTK) antibody (ABIN968992) Figure 2: Immunohistochemical analysis of paraffin-embedded human lymph-node tissues ...
Western Blotting (WB) image for anti-Bruton Agammaglobulinemia tyrosine Kinase (BTK) antibody (ABIN968992) Figure 3: Western blot analysis using BTK mouse mAb against K562 (1), MCF-7 (2), Jurk...
Background publications Yamada, Kawakami, Kimura et al.: "Structure and expression of novel protein-tyrosine kinases, Emb and Emt, in hematopoietic cells." in: Biochemical and biophysical research communications, Vol. 192, Issue 1, pp. 231-40, 1993 (PubMed).

Thomas, Sideras, Smith et al.: "Colocalization of X-linked agammaglobulinemia and X-linked immunodeficiency genes." in: Science (New York, N.Y.), Vol. 261, Issue 5119, pp. 355-8, 1993 (PubMed).

Tamagnone, Lahtinen, Mustonen et al.: "BMX, a novel nonreceptor tyrosine kinase gene of the BTK/ITK/TEC/TXK family located in chromosome Xp22.2." in: Oncogene, Vol. 9, Issue 12, pp. 3683-8, 1994 (PubMed).