BTK antibody (Bruton Agammaglobulinemia tyrosine Kinase)

Details for Product anti-BTK Antibody No. ABIN968992, Supplier: Log in to see
Antigen
  • BTK
  • atk
  • bpk
  • xla
  • imd1
  • agmx1
  • psctk1
  • AGMX1
  • AT
  • ATK
  • BPK
  • IMD1
  • PSCTK1
  • XLA
  • AI528679
  • xid
  • Bruton agammaglobulinemia tyrosine kinase
  • BTK
  • btk
  • Btk
Alternatives
anti-Monkey BTK antibody for ELISA
Reactivity
Human, Monkey
286
128
68
18
16
8
6
3
Host
Mouse
195
91
Clonality (Clone)
Monoclonal ()
Conjugate
This BTK antibody is un-conjugated
12
12
9
6
5
5
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
224
111
106
82
75
41
40
37
10
10
5
3
2
2
Options
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Immunogen Purified recombinant fragment of BTK expressed in E. Coli.
Clone 7F12H4
Isotype IgG1
Purification Ascites
Alternative Name BTK (BTK Antibody Abstract)
Background Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases.All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini.BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation.
Molecular Weight 77 kDa
Gene ID 695
HGNC 695
Research Area Immunology, Innate Immunity, Tyrosine Kinases
Pathways Fc-epsilon Receptor Signaling Pathway, Hormone Transport, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage
Supplier Images
Immunocytochemistry (ICC) image for anti-BTK antibody (Bruton Agammaglobulinemia tyrosine Kinase) (ABIN968992) Figure 1: Immunofluorescence analysis of Jurkat cells using BTK mouse mAb.
Immunohistochemistry (IHC) image for anti-BTK antibody (Bruton Agammaglobulinemia tyrosine Kinase) (ABIN968992) Figure 2: Immunohistochemical analysis of paraffin-embedded human lymph-node tissues ...
Western Blotting (WB) image for anti-BTK antibody (Bruton Agammaglobulinemia tyrosine Kinase) (ABIN968992) Figure 3: Western blot analysis using BTK mouse mAb against K562 (1), MCF-7 (2), Jurk...
Background publications Tamagnone, Lahtinen, Mustonen, Virtaneva, Francis, Muscatelli, Alitalo, Smith, Larsson, Alitalo: "BMX, a novel nonreceptor tyrosine kinase gene of the BTK/ITK/TEC/TXK family located in chromosome Xp22.2." in: Oncogene, Vol. 9, Issue 12, pp. 3683-8, 1994 (PubMed).

Yamada, Kawakami, Kimura, Fukamachi, Baier, Altman, Kato, Inagaki, Kawakami: "Structure and expression of novel protein-tyrosine kinases, Emb and Emt, in hematopoietic cells." in: Biochemical and biophysical research communications, Vol. 192, Issue 1, pp. 231-40, 1993 (PubMed).

Thomas, Sideras, Smith, Vorechovský, Chapman, Paul: "Colocalization of X-linked agammaglobulinemia and X-linked immunodeficiency genes." in: Science (New York, N.Y.), Vol. 261, Issue 5119, pp. 355-8, 1993 (PubMed).