Nuclear Factor-KB P65 (NFkBP65) antibody

Details for Product No. ABIN968997
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Antigen
Synonyms c-Rel, C-Rel, Xrel2, c-rel, rel-A, v-rel, xrel, NFKB3, p65, NFkB, zgc:100833
Reactivity
Human, Mouse (Murine)
(899), (445), (356), (76), (75), (74), (38), (9), (8), (6), (2), (2), (1), (1)
Host
Mouse
(842), (68), (6), (5), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(24), (19), (15), (11), (10), (10), (10), (10), (10), (10), (10), (10), (10), (10), (1), (1), (1)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
(689), (364), (299), (186), (151), (126), (100), (54), (38), (21), (19), (19), (18), (12), (7), (5), (2), (1)
Pubmed 2 references available
Quantity 100 μL
Options
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Catalog No. ABIN968997
396.00 $
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Immunogen Purified recombinant fragment of human c-Rel expressed in E. coli.
Clone 1-00E-07
Isotype IgG1
Purification Ascites
Alternative Name c-Rel
Background Synonyms:
Rel, c-Rel
The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA (MIM 164014), RELB (604758), NFKB1 (MIM 164011), and NFKB2 (MIM 164012). These proteins are related through a highly conserved N-terminal region termed the 'Rel domain,' which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor.
Molecular Weight 78 kDa
Gene ID 5966
Research Area Cancer, Transcription Factors
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-Nuclear Factor-KB P65 (NFkBP65) antibody Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 ng), Blue: Antigen (100 ng)
anti-Nuclear Factor-KB P65 (NFkBP65) antibody (2) Figure 3: Immunofluorescence analysis of U251 cells using c-Rel mouse mAb (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
anti-Nuclear Factor-KB P65 (NFkBP65) antibody (3) Figure 2: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues (left) and liver cancer tissues (right) using c-Rel mouse mAb with DAB staining.
anti-Nuclear Factor-KB P65 (NFkBP65) antibody (4) Figure 1: Western blot analysis using c-Rel mouse mAb against Jurkat (1), NIH/3T3 (2), Hela (3), HEK293 (4) and RAJI (5) cell lysate.
Product cited in: Garbati, Gilmore: "Ser484 and Ser494 in REL are the major sites of IKK phosphorylation in vitro: evidence that IKK does not directly enhance GAL4-REL transactivation." in: Gene expression, Vol. 14, Issue 4, pp. 195-205, 2008 (PubMed).

Trynka, Zhernakova, Romanos et al.: "Coeliac disease-associated risk variants in TNFAIP3 and REL implicate altered NF-kappaB signalling." in: Gut, Vol. 58, Issue 8, pp. 1078-83, 2009 (PubMed).

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