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Chaperonin Containing TCP1, Subunit 2 (Beta) (CCT2) antibody

Details for Product No. ABIN969010, Supplier: Log in to see
Antigen
  • fa26a01
  • fc58a11
  • CHUNP6883
  • wu:fa26a01
  • wu:fc58a11
  • cct2
  • MGC89448
  • CCT2
  • 99D8.1
  • CCT-beta
  • CCTB
  • PRO1633
  • TCP-1-beta
  • Cctb
Reactivity
Human, Mouse (Murine), Monkey, Rat (Rattus)
62
34
27
7
5
5
5
5
5
4
3
2
2
1
1
1
1
1
1
Host
Mouse
34
22
12
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
57
17
13
11
11
9
3
2
2
2
1
1
1
1
Supplier
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Immunogen Purified recombinant fragment of human CCT2 expressed in E. coli.
Clone 5B5C4
Isotype IgG1
Purification Ascites
Alternative Name CCT2 (CCT2 Antibody Abstract)
Background The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Two transcript variants encoding different isoforms have been found for this gene.
Molecular Weight 54 kDa
Gene ID 10576
HGNC 10576
Pathways
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
ELISA image for anti-Chaperonin Containing TCP1, Subunit 2 (Beta) (CCT2) antibody (ABIN969010) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-Chaperonin Containing TCP1, Subunit 2 (Beta) (CCT2) antibody (ABIN969010) Figure 2: Flow cytometric analysis of NIH/3T3 cells using CCT2 mouse mAb (blue) and n...
Immunocytochemistry (ICC) image for anti-Chaperonin Containing TCP1, Subunit 2 (Beta) (CCT2) antibody (ABIN969010) Figure 3: Immunofluorescence analysis of 3T3-L1 cells using CCT2 mouse mAb (green) . ...
Western Blotting (WB) image for anti-Chaperonin Containing TCP1, Subunit 2 (Beta) (CCT2) antibody (ABIN969010) Figure 4: Western blot analysis using CCT2 mouse mAb against Hela (1), MCF-7 (2), Jur...
Western Blotting (WB) image for anti-Chaperonin Containing TCP1, Subunit 2 (Beta) (CCT2) antibody (ABIN969010) Figure 5: Western blot analysis using CCT2 mAb against human CCT2 (AA: 87-290) recomb...
Product cited in: Abe, Yoon, Kubota et al.: "p90 ribosomal S6 kinase and p70 ribosomal S6 kinase link phosphorylation of the eukaryotic chaperonin containing TCP-1 to growth factor, insulin, and nutrient signaling." in: The Journal of biological chemistry, Vol. 284, Issue 22, pp. 14939-48, 2009 (PubMed).

Goudreault, DAmbrosio, Kean et al.: "A PP2A phosphatase high density interaction network identifies a novel striatin-interacting phosphatase and kinase complex linked to the cerebral cavernous malformation 3 (CCM3) protein." in: Molecular & cellular proteomics : MCP, Vol. 8, Issue 1, pp. 157-71, 2009 (PubMed).