CDC27 antibody (Cell Division Cycle 27 Homolog (S. Cerevisiae))

Details for Product anti-CDC27 Antibody No. ABIN969035, Supplier: Log in to see
Antigen
  • CDC27
  • NV10210
  • ANAPC3
  • APC3
  • CDC27Hs
  • D0S1430E
  • D17S978E
  • HNUC
  • NUC2
  • AI452358
  • BC023187
  • cell division cycle 27 homolog (S. cerevisiae)
  • cell division cycle 27
  • CDC27
  • cdc27
  • Cdc27
Reactivity
Human
117
54
47
23
18
14
13
7
6
4
3
3
3
2
2
1
Host
Mouse
91
44
Clonality (Clone)
Monoclonal ()
Conjugate
This CDC27 antibody is un-conjugated
4
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
101
61
36
27
24
13
11
7
3
3
1
1
1
Options
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Immunogen Purified recombinant fragment of human CDC27 expressed in E. Coli.
Clone 5C12
Isotype IgG1
Purification Ascites
Alternative Name CDC27 (CDC27 Antibody Abstract)
Background Cdc27 shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. It is a component of the Anaphase Promoting Complex (APC), which is composed of eight protein subunits and is highly conserved in eucaryotic cells. The APC catalyzes the formation of the cyclin B ubiquitin conjugate that is responsible for the ubiquitin mediated proteolysis of B type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.
Molecular Weight 91 kDa
Gene ID 996
HGNC 996
Research Area CD Antigens, Surface Receptors of Immune Cells, Cell Cycle, Coagulation
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage
Supplier Images
Immunohistochemistry (IHC) image for anti-CDC27 antibody (Cell Division Cycle 27 Homolog (S. Cerevisiae)) (ABIN969035) Figure 1: Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left...
Western Blotting (WB) image for anti-CDC27 antibody (Cell Division Cycle 27 Homolog (S. Cerevisiae)) (ABIN969035) Figure 2: Western blot analysis using CDC27 mouse mAb against CDC27 (AA: 724-830) -hI...
Product cited in: Goto, Eddy: "Speriolin is a novel spermatogenic cell-specific centrosomal protein associated with the seventh WD motif of Cdc20." in: The Journal of biological chemistry, Vol. 279, Issue 40, pp. 42128-38, 2004 (PubMed).

Koloteva-Levine, Pinchasi, Pereman, Zur, Brandeis, Elroy-Stein: "The Apc5 subunit of the anaphase-promoting complex/cyclosome interacts with poly(A) binding protein and represses internal ribosome entry site-mediated translation." in: Molecular and cellular biology, Vol. 24, Issue 9, pp. 3577-87, 2004 (PubMed).