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ER-A antibody

Details for Product No. ABIN969110, Supplier: Log in to see
Antigen
Reactivity
Human
2
Host
Mouse
1
Clonality (Clone)
Monoclonal ()
Application
ELISA, Western Blotting (WB)
2
1
Supplier
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Immunogen Purified recombinant fragment of ER expressed in E. coli.
Clone 5D4B1
Isotype IgG2b
Purification Ascites
Alternative Name ER
Background ER (estrogen receptor 1) a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery. Phosphorylation provides an important mechanism to regulate ER activity. ER is phosphorylated on multiple sites. Serines 104, 106, 118 and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serines plays an important role in regulating ER activity. Ser118 may be the substrate of the transcription regulatory kinase cdK7. Ser167 may be phosphorylated by p90RSK and Akt. Phosphorylation of Ser167 may confer tamoxifen resistance in breast cancer patients.
Gene ID 2099
HGNC 2099
Pathways
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
Western Blotting (WB) image for anti-ER-A antibody (ABIN969110) Figure 1: Western blot analysis using ER mouse mAb truncated ER recombinant protein (...
Western Blotting (WB) image for anti-ER-A antibody (ABIN969110) Figure 2: Western blot analysis using ER mouse mAb against HEK293T cells transfected ...
Product cited in: Wang, Li, Chen: "[Anatomical study and clinical applications of the tensor fascia lata myocutaneous flap]." in: Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery, Vol. 16, Issue 1, pp. 12-3, 2001 (PubMed).

Campbell, Bhat-Nakshatri, Patel et al.: "Phosphatidylinositol 3-kinase/AKT-mediated activation of estrogen receptor alpha: a new model for anti-estrogen resistance." in: The Journal of biological chemistry, Vol. 276, Issue 13, pp. 9817-24, 2001 (PubMed).

Chen, Riedl, Washbrook et al.: "Activation of estrogen receptor alpha by S118 phosphorylation involves a ligand-dependent interaction with TFIIH and participation of CDK7." in: Molecular cell, Vol. 6, Issue 1, pp. 127-37, 2000 (PubMed).