Integrin beta 3 (ITGB3) antibody

Antigen: Integrin beta 3 (ITGB3)

Synonyms: LAD, CD18, MF17, MFI7, LCAMB, LFA-1, MAC-1, CD61, GP3A, GPIIIa, INGRB3, MGC159221, MGC159223, integrin, ITGB3

Clonality: Monoclonal (Y2-51)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN96913

Additional Information

Alternative name: CD61

Immunogen: BALB/C mice were injected with PHA stimulated human mononuclear cells.

Format: Supernatant (prediluted)

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: Y2-51

Specificity: This antibody recognizes a glycoprotein of 110 kD molecular weight present on human platelets and also reacts with megakaryocytes and megakaryoblasts. The antigen is also present on endothelium and osteoclasts. The glycoprotein IIIa molecule produced by endothelium cells is identical in sequence to platelet glycoprotein IIIa.

Application Details

Application Notes: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. This antibody is not compatible with Zenker and B5 fixed tissues. Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane

Concentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Storage: Store the antibody 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: CD Antigens

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Zimrin, Eisman, Vilaire et al.: "Structure of platelet glycoprotein IIIa. A common subunit for two different membrane receptors." in: The Journal of clinical investigation, Vol. 81, Issue 5, pp. 1470-5, 1988 (PubMed).

Kanz, Mielke, Fauser: "Analysis of human hemopoietic progenitor cells for the expression of glycoprotein IIIa." in: Experimental hematology, Vol. 16, Issue 9, pp. 741-7, 1988 (PubMed).

Gatter, Cordell, Turley et al.: "The immunohistological detection of platelets, megakaryocytes and thrombi in routinely processed specimens." in: Histopathology, Vol. 13, Issue 3, pp. 257-67, 1989 (PubMed).