IHOG antibody
(1 reference)-
- Target See all IHOG products
- IHOG (Interference Hedgehog (IHOG))
- Reactivity
- Drosophila melanogaster
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- ELISA, Western Blotting (WB)
- Purification
- purified
- Immunogen
- Purified recombinant fragment of human IHOG expressed in E. coli.
- Clone
- 3G8
- Isotype
- IgG1
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- Application Notes
- ELISA: 1:10000, WB: 1:500 - 1:2000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Ascitic fluid containing 0.03 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
- 4°C, -20°C for long term storage
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-
: "Structure of a heparin-dependent complex of Hedgehog and Ihog." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 46, pp. 17208-13, (2006) (PubMed).
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: "Structure of a heparin-dependent complex of Hedgehog and Ihog." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 46, pp. 17208-13, (2006) (PubMed).
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- Target
- IHOG (Interference Hedgehog (IHOG))
- Alternative Name
- IHOG
- Synonyms
- CG9211 antibody, CT26314 antibody, Dmel\\CG9211 antibody, Ihog antibody, iHog antibody, interference hedgehog antibody, ihog antibody
- Background
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Description: The ihog gene (interference hedgehog), identified by RNA interference in Drosophila cultured cells, encodes a type 1 membrane protein shown here to bind and to mediate response to the active Hedgehog (Hh) protein signal. ihog mutations produce defects characteristic of Hh signaling loss in embryos and imaginal discs, and epistasis analysis places ihog action at or upstream of the negatively acting receptor component, Patched (Ptc). The first of two extracellular fibronectin type III (FNIII) domains of the Ihog protein mediates a specific interaction with Hh protein in vitro, but the second FNIII domain is additionally required for in vivo signaling activity and for Ihog-enhanced binding of Hh protein to cells coexpressing Ptc. Other members of the Ihog family, including Drosophila Boi and mammalian CDO and BOC, also interact with Hh ligands via a specific FNIII domain, thus identifying an evolutionarily conserved family of membrane proteins that function in Hh signal response.
Aliases: CG9211, CT26314, Dmel\CG9211, ihog, Ihog
- Molecular Weight
- 98 kDa
- Gene ID
- 33972
- HGNC
- 33972
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