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Interference Hedgehog (IHOG) antibody

Details for Product No. ABIN969211
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Antigen
Synonyms CG9211, CT26314, Dmel\\CG9211, Ihog, iHog
Reactivity
Fruit Fly (Drosophila melanogaster)
(2), (1), (1)
Host
Mouse
(5)
Clonality (Clone)
Monoclonal ()
Application
ELISA, Western Blotting (WB)
(5), (4)
Pubmed 1 reference available
Quantity 100 µL
Options
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Availability Will be delivered in 3 to 4 Business Days
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Catalog No. ABIN969211
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Immunogen Purified recombinant fragment of human IHOG expressed in E. coli.
Clone 3G8
Isotype IgG1
Purification Ascites
Alternative Name IHOG
Background Synonyms:
CG9211, CT26314, Dmel\\\\\\\\\\\\\\\\CG9211, ihog, Ihog
The ihog gene (interference hedgehog), identified by RNA interference in Drosophila cultured cells, encodes a type 1 membrane protein shown here to bind and to mediate response to the active Hedgehog (Hh) protein signal. ihog mutations produce defects characteristic of Hh signaling loss in embryos and imaginal discs, and epistasis analysis places ihog action at or upstream of the negatively acting receptor component, Patched (Ptc). The first of two extracellular fibronectin type III (FNIII) domains of the Ihog protein mediates a specific interaction with Hh protein in vitro, but the second FNIII domain is additionally required for in vivo signaling activity and for Ihog-enhanced binding of Hh protein to cells coexpressing Ptc. Other members of the Ihog family, including Drosophila Boi and mammalian CDO and BOC, also interact with Hh ligands via a specific FNIII domain, thus identifying an evolutionarily conserved family of membrane proteins that function in Hh signal response.
Molecular Weight 98 kDa
Gene ID 33972
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-Interference Hedgehog (IHOG) antibody Figure 1: Western blot analysis using IHOG mAb against IHOG-hIgGFc transfected HEK293 cell lysate.
Product cited in: McLellan, Yao, Zheng et al.: "Structure of a heparin-dependent complex of Hedgehog and Ihog." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 46, pp. 17208-13, 2006 (PubMed).

Validation Images
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