Selectin E (SELE) antibody
| Antigen |
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| Synonyms |
ELAM, ESEL, CD62E, ELAM1, LECAM2, SELE |
| Clonality |
Monoclonal (1-2B6) |
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Host
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Reactivity
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Application
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Flow Cytometry (FACS) (32), Western Blotting (WB) (22), Immunohistochemistry (Frozen Sections) (IHC (fro)) (16), Immunoprecipitation (IP) (16), ELISA (12), Immunohistochemistry (IHC) (11), Functional Studies (Func) (9), Immunocytochemistry (ICC) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), ELISA (Detection) (3), Enzyme Immunoassay (EIA) (3), Blocking Antibody (Inhibition) (2), Immunofluorescence (IF) (1)
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| Catalog no. |
ABIN96927 |
| Quantity |
1ml (100ug/ml approximately) (Variants) |
| Price |
451.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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CD62E
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Immunogen
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BALB/C mice were injected with TNF-activated human umbilical vein endothelial cells.
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Format
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Supernatant
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Isotype
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IgG1 kappa (Matching secondary antibodies)
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Clone
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1-2B6
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Specificity
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This antibody is specific to E-selectin in human and pigs. E- selectin is a single-chain glycoprotein of 110 kD and is expressed by activated endothelial cells. E-selectin functions as an adhesion molecule for leukocytes in inflammation.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. This antibody is suitable for frozen tissue sections only. Positive Control: Tonsil. Cellular localization: Cell membrane
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as purified immunoglobulin containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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CD Antigens
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Publications
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Corkill, Kirkham, Haskard et al.: "Gold treatment of rheumatoid arthritis decreases synovial expression of the endothelial leukocyte adhesion receptor ELAM-1." in: The Journal of rheumatology, Vol. 18, Issue 10, pp. 1453-60, 1992 (PubMed).
Bevilacqua, Nelson: "Selectins." in: The Journal of clinical investigation, Vol. 91, Issue 2, pp. 379-87, 1993 (PubMed).
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Alternatives for antigen "Selectin E (SELE)", type "Antibodies"
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Hosts
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Mouse (30), Rat (15), Rabbit (6), Sheep (1)
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Reactivities
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Human (43), Mouse (Murine) (16), Rat (Rattus) (4)
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Applications
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Flow Cytometry (FACS) (32), Western Blotting (WB) (22), Immunohistochemistry (Frozen Sections) (IHC (fro)) (16), Immunoprecipitation (IP) (16), ELISA (12), Immunohistochemistry (IHC) (11), Functional Studies (Func) (9), Immunocytochemistry (ICC) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), ELISA (Detection) (3), Enzyme Immunoassay (EIA) (3), Blocking Antibody (Inhibition) (2), Immunofluorescence (IF) (1)
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Conjugates
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FITC (6), Biotin (3), PE (3), APC (1), SPRD (1)
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Epitopes
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CR1 (2), CR2 (1)
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Alternatives 
