CD73 antibody

Catalog No. ABIN969322

Product Details anti-CD73 Antibody

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD73 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Purification: purified

Immunogen: Purified recombinant fragment of NT5E expressed in E. coli.

Clone: 1D7

Isotype: IgG1

Application Details

Application Notes: ELISA: 1:10000, IHC: 1:200 - 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: 4°C, -20°C for long term storage

References for anti-CD73 antibody (ABIN969322)

Zhou, Zhi, Zhou, Chen, Zhao, Shao, Ou, Yin: "Effects of ecto-5'-nucleotidase on human breast cancer cell growth in vitro and in vivo." in: Oncology reports, Vol. 17, Issue 6, pp. 1341-6, (2007) (PubMed).

Wink, Braganhol, Tamajusuku, Casali, Karl, Barreto-Chaves, Sarkis, Battastini: "Extracellular adenine nucleotides metabolism in astrocyte cultures from different brain regions." in: Neurochemistry international, Vol. 43, Issue 7, pp. 621-8, (2003) (PubMed).

Target Details for CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: NT5E (NT5E Products)

Synonyms: CALJA antibody, CD73 antibody, E5NT antibody, NT antibody, NT5 antibody, NTE antibody, eN antibody, eNT antibody, zgc:63784 antibody, NT5E antibody, MGC114869 antibody, PSPTO2277 antibody, 2210401F01Rik antibody, AI447961 antibody, Nt5 antibody, 5'-nucleotidase ecto antibody, 5'-nucleotidase, ecto (CD73) antibody, 5'-nucleotidase antibody, 5' nucleotidase, ecto antibody, NT5E antibody, nt5e antibody, nt5e.S antibody, PSPTO_2277 antibody, CBU_1233 antibody, MCA1322 antibody, Nt5e antibody

Background:

Description: 5'-nucleotidase, ecto (NT5E), also known as CD73 (Cluster of Differentiation 73). Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase, EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.

Aliases: eN, NT5, CD73

Molecular Weight: 70 kDa

Gene ID: 4907

HGNC: 4907

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process