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Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) antibody

Details for Product No. ABIN969325, Supplier: Log in to see
Antigen
  • B23
  • B23NP
  • CG7917
  • Crp1
  • CRP1
  • Dmel\\CG7917
  • dNLP
  • dNLP-S
  • NO38
  • Npm
  • npm
  • NPM
  • npm4
  • p22
Reactivity
Human, Monkey
337
131
128
10
10
10
10
7
3
2
2
1
1
1
1
Host
Mouse
232
119
17
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
15
13
9
6
6
6
6
4
4
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
292
167
106
95
79
38
37
27
26
7
7
5
3
Supplier
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Immunogen Purified recombinant fragment of human NPM (AA 2-265) expressed in E. coli.
Clone 7H10B9
Isotype IgG1
Purification Ascites
Alternative Name Nucleophosmin (NPM1 Antibody Abstract)
Background Nucleophosmin (NPM), also named B23 or NO38, is a major nucleolar protein which is 20 times more abundant in tumor or proliferating cells than in normal resting cells. NPM has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication and molecular chaperoning activities. The NPM is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase.
Molecular Weight 33 kDa
Gene ID 4869
HGNC 4869
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
Immunocytochemistry (ICC) image for anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) antibody (ABIN969325) Figure 1: Confocal immunofluorescence analysis of Hela (left) and NTERA-2 (right) cel...
Immunohistochemistry (IHC) image for anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) antibody (ABIN969325) Figure 2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tis...
Western Blotting (WB) image for anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) antibody (ABIN969325) Figure 3: Western blot analysis using NPM mouse mAb against SMMC-7721 (1), HepG2 (2),...
Background publications Yoneda-Kato, Look, Kirstein, Valentine, Raimondi, Cohen, Carroll, Morris: "The t(3;5)(q25.1;q34) of myelodysplastic syndrome and acute myeloid leukemia produces a novel fusion gene, NPM-MLF1." in: Oncogene, Vol. 12, Issue 2, pp. 265-75, 1996 (PubMed).

Product cited in: Morris, Kirstein, Valentine, Dittmer, Shapiro, Saltman, Look: "Fusion of a kinase gene, ALK, to a nucleolar protein gene, NPM, in non-Hodgkin's lymphoma." in: Science (New York, N.Y.), Vol. 263, Issue 5151, pp. 1281-4, 1994 (PubMed).