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RAB25, Member RAS Oncogene Family (RAB25) antibody

Details for Product No. ABIN969377
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Antigen
Synonyms CATX-8, RAB11C, AV012978, MGC84182, RAB25, catx-8, MGC79587, rab25, si:dkeyp-84f11.7, zgc:101648
Reactivity
Human, Mouse (Murine)
(44), (19), (13), (13), (13), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Mouse
(25), (16), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Fluorescence Microscopy (FM)
(35), (23), (13), (13), (10), (7), (5), (3), (1), (1)
Pubmed 1 reference available
Quantity 100 µL
Options
Shipping to United States (Change)
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Catalog No. ABIN969377
363.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Purified recombinant fragment of Rab25 expressed in E. coli.
Clone 3F12
Isotype IgG1
Purification Ascites
Alternative Name Rab25
Background Synonyms:
CATX-8, RAB25
Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50 % similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40 % similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric pariηl cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa.
Molecular Weight 24 kDa
Gene ID 57111
Research Area Cancer
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-RAB25, Member RAS Oncogene Family (RAB25) antibody Figure 3: Flow cytometric analysis of NIH/3T3 cells using Rab25 mouse mAb (green) and negative control (purple) .
anti-RAB25, Member RAS Oncogene Family (RAB25) antibody (2) Figure 4: Immunofluorescence analysis of A549 cells using RAB25 mouse mAb (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
anti-RAB25, Member RAS Oncogene Family (RAB25) antibody (3) Figure 2: Immunohistochemical analysis of paraffin-embedded esophagus tissues (left) and human lung cancer (right) using Rab25 mouse mAb with DAB staining.
anti-RAB25, Member RAS Oncogene Family (RAB25) antibody (4) Figure 1: Western blot analysis using Rab25 mouse mAb against MCF-7 (1), T47D (2) and GC7901 (3) cell lysate.
Product cited in: Goldenring, Shen, Vaughan et al.: "Identification of a small GTP-binding protein, Rab25, expressed in the gastrointestinal mucosa, kidney, and lung." in: The Journal of biological chemistry, Vol. 268, Issue 25, pp. 18419-22, 1993 (PubMed).

Hosts (25), (16), (3)
Reactivities (44), (19), (13), (13), (13), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applications (35), (23), (13), (13), (10), (7), (5), (3), (1), (1)
Conjugates (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (8), (7), (2), (2), (2)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN969377
363.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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